The Effectiveness Of The Application Of Oral Wound Dressing Films That Contain Chitosan To The Number Of Macrophages After The Gingival Incision In Wistar Mice (Rattus Norvegicus)

Feb 27, 2025 by ADMIN 180 views

The Effectiveness of the Application of Oral Wound Dressing Films that Contain Chitosan to the Number of Macrophages after the Gingival Incision in Wistar Mice (Rattus norvegicus)

Introduction

Periodontal surgical treatment is a common procedure that involves opening or exposing parts of the body's tissues, which can lead to open or closed wounds. These wounds can cause tissue tears, bleeding, swelling, infection, and scarring if not treated properly. Therefore, periodontal dressing is necessary to cover the wound and minimize the risk of infection. Traditional periodontal packaging available today is generally based on chemicals, which can cause discomfort when used and disrupt aesthetic aspects.

The Need for Alternative Periodontal Dressings

As an alternative to traditional periodontal packaging, the use of film dressing can be considered for oral wounds. This type of dressing is considered more aesthetic, comfortable to use, and more easily applied to the surface of the tissue due to its adhesive and flexible nature. Film dressing not only protects the surface of the wound but can also reduce pain and control bleeding. One of the promising ingredients for use in film dressing is chitosan, which has mucoadhesive, bioactive, biocompatible, biodegradable, antimicrobial, and non-toxic traits compared to other polymers. Chitosan also functions as an anti-inflammatory, hemostatic agent, and as a good drug transport, and can stimulate cell proliferation and tissue reshuffle.

The Role of Macrophages in Wound Healing

Macrophages play a crucial role in the wound healing process. They function in immune responses and tissue cleaning dead cells, thereby accelerating tissue regeneration. The increase in the number of macrophages on the 3rd day after the incision and a decrease in the number of macrophages on the 7th and 14th days indicate that the oral wounds based on chitosan are effective in accelerating the process of healing wounds after the gingival incision in wistar mice.

The Effectiveness of Chitosan-Based Film Dressing

The results of this study showed that oral wounds based on chitosan were effective in accelerating the process of healing wounds after the gingival incision in wistar mice. This can be seen from the increase in the number of macrophages on the 3rd day after the incision and a decrease in the number of macrophages on the 7th and 14th days. The use of chitosan-based film dressing can be a better choice than traditional treatment methods. In addition to providing higher comfort and safety for patients, this product also shows great potential in increasing the efficiency of periodontal wound healing.

Conclusion

Overall, the use of movie dressing wounds that contain chitosan can be a better choice than traditional treatment methods. In addition to providing higher comfort and safety for patients, this product also shows great potential in increasing the efficiency of periodontal wound healing. Thus, further research is needed to explore clinical applications from this chitosan film dressing and its impact on human patients.

Materials and Methods

This study is an experimental study in Vivo laboratory with post-test control group design that measures clinical parameters using landry healing index and histological parameters by observing the number of macrophages. The sample consists of 45 healthy male wistar mice aged 2-3 months.

Results

The results showed that oral wounds based on chitosan -based wounds were effective in accelerating the process of healing wounds after the gingival incision in wistar mice. This can be seen from the increase in the number of macrophages on the 3rd day after the incision and a decrease in the number of macrophages on the 7th and 14th days.

Discussion

The increase in the number of macrophages on the 3rd day after the incision and a decrease in the number of macrophages on the 7th and 14th days indicate that the oral wounds based on chitosan are effective in accelerating the process of healing wounds after the gingival incision in wistar mice. Macrophages play a crucial role in the wound healing process. They function in immune responses and tissue cleaning dead cells, thereby accelerating tissue regeneration.

Conclusion

Recommendations

Based on the results of this study, it is recommended that further research be conducted to explore clinical applications from this chitosan film dressing and its impact on human patients. Additionally, the use of chitosan-based film dressing should be considered as a better choice than traditional treatment methods for periodontal wound healing.

Limitations

This study has several limitations. The sample size is relatively small, and the study was conducted in a laboratory setting. Therefore, further research is needed to confirm the results of this study and to explore clinical applications from this chitosan film dressing.

Future Directions

Future research should focus on exploring clinical applications from this chitosan film dressing and its impact on human patients. Additionally, the use of chitosan-based film dressing should be considered as a better choice than traditional treatment methods for periodontal wound healing.

References

[List of references cited in the study]

Appendix

[List of appendices, including any additional data or information that may be relevant to the study]
Q&A: The Effectiveness of Oral Wound Dressing Films that Contain Chitosan

Q: What is the purpose of this study?

A: The purpose of this study is to determine the effectiveness of oral wound dressing films that contain chitosan in increasing the number of macrophages during the wound healing process after the gingival incision in wistar mice.

Q: What is chitosan and why is it used in wound dressing films?

A: Chitosan is a biodegradable and biocompatible polymer that has mucoadhesive, bioactive, and antimicrobial properties. It is used in wound dressing films because it can stimulate cell proliferation and tissue reshuffle, making it an effective ingredient for wound healing.

Q: What is the role of macrophages in wound healing?

A: Macrophages play a crucial role in the wound healing process. They function in immune responses and tissue cleaning dead cells, thereby accelerating tissue regeneration.

Q: What were the results of this study?

A: The results of this study showed that oral wounds based on chitosan were effective in accelerating the process of healing wounds after the gingival incision in wistar mice. This can be seen from the increase in the number of macrophages on the 3rd day after the incision and a decrease in the number of macrophages on the 7th and 14th days.

Q: What are the benefits of using chitosan-based wound dressing films?

A: The benefits of using chitosan-based wound dressing films include higher comfort and safety for patients, increased efficiency of periodontal wound healing, and the potential to reduce the risk of infection.

Q: What are the limitations of this study?

A: The limitations of this study include a relatively small sample size and the fact that it was conducted in a laboratory setting. Therefore, further research is needed to confirm the results of this study and to explore clinical applications from this chitosan film dressing.

Q: What are the future directions for this research?

A: Future research should focus on exploring clinical applications from this chitosan film dressing and its impact on human patients. Additionally, the use of chitosan-based film dressing should be considered as a better choice than traditional treatment methods for periodontal wound healing.

Q: What are the potential applications of this research?

A: The potential applications of this research include the development of new wound dressing films that contain chitosan, which can be used to treat various types of wounds, including periodontal wounds.

Q: What are the implications of this research for the field of periodontics?

A: The implications of this research for the field of periodontics include the potential to develop new and more effective treatments for periodontal wounds, which can improve patient outcomes and reduce the risk of complications.

Q: What are the next steps for this research?

A: The next steps for this research include conducting further studies to confirm the results of this study and to explore clinical applications from this chitosan film dressing. Additionally, the development of new wound dressing films that contain chitosan should be considered as a potential application of this research.

Q: What are the potential benefits of using chitosan-based wound dressing films in clinical practice?

A: The potential benefits of using chitosan-based wound dressing films in clinical practice include improved patient outcomes, reduced risk of complications, and increased efficiency of wound healing.

Q: What are the potential risks or side effects of using chitosan-based wound dressing films?

A: The potential risks or side effects of using chitosan-based wound dressing films include allergic reactions, irritation, or other adverse effects. However, these risks are generally considered to be low, and further research is needed to confirm the safety of this treatment.

Q: What are the potential future directions for this research?

A: The potential future directions for this research include exploring the use of chitosan-based wound dressing films in other types of wounds, such as burns or surgical wounds, and investigating the potential benefits of using chitosan-based wound dressing films in combination with other treatments.