The Effect Of Puguntano Herbal Water Extract (picria Felt-terrae Lour.) Against A Decrease In Blood Glucose Levels Induced By Streptozotocin

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The Effect of Puguntano Herbal Water Extract (Picria Fel-Terrae Lour.) Against a Decrease in Blood Glucose Levels Induced by Streptozotocin

Introduction

Diabetes Mellitus (DM) is a major cause of death in many countries, requiring long-term care that often comes with a high cost. The search for affordable and effective antidiabetic drugs is increasingly important. One promising alternative is research on medicinal plants that have antidiabetic effects. Puguntano (Picria Fel-Terrae Lour.) is one of the plants used for this purpose. This article will discuss research conducted to evaluate the effect of decreasing blood glucose levels from Puguntano Herbal Water Extract (EAHP) on male mice induced by streptozotocin.

Background

Diabetes Mellitus (DM) is a chronic metabolic disorder characterized by high blood glucose levels, which can lead to various complications such as cardiovascular disease, kidney failure, and nerve damage. The increasing prevalence of DM has made it a major public health concern worldwide. The traditional treatment for DM involves the use of insulin and oral hypoglycemic agents, which can have side effects and are often expensive. Therefore, there is a need to explore alternative treatments that are safe, effective, and affordable.

Methodology

In this study, Puguntano extract was obtained through the infundation method, then concentrated using a rotary evaporator so as to produce a thick extract. This study involved 24 male mice divided into six treatment groups. The negative control group receives 0.5% Na-CMC with a dose of 1% body weight, while the treatment group receives EAHP at a dose of 200, 400, and 800 mg/kg body weight. The positive control group receives metformin at a dose of 65 mg/kg body weight, and the normal control group does not receive any treatment. Mice are induced with streptozotocin (STZ) with a dose of 55 mg/kg body weight intraperitoneal, then treatment for 15 days. Measurement of blood glucose levels is carried out on days 0, 3rd, 5th, 7th, 9th, 11th, 13th, and 15th. After that, a pancreas histology examination is carried out by the hematoxylin eosin (HE) coloring method.

Results

The results of the analysis show that the administration of EAHP significantly reduces blood glucose levels in mice compared to negative control groups that receive Na-CMC 0.5% (p = 0.000). The most significant decrease in glucose levels occurs in groups that receive a dose of 200 mg/kg of body weight, with an average blood glucose level on the 15th day of 82.25 mg/dl. On the other hand, groups with a dose of 800 mg/kg body weight show an average blood glucose level of 91.50 mg/dl, while groups with a dose of 400 mg/kg body weight reaches 93.75 mg/dl. Further statistical analysis shows that glucose levels in the three doses of EAHP (200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW) are not significantly different from the positive control group that receives metformin (p = 0.986, p = 0.065, p = 0.235).

Discussion

The results of this study suggest that Puguntano Herbal Water Extract (EAHP) has the potential as a blood glucose-lowering agent and can contribute to prevent further damage to the pancreas tissue in mice with diabetes induced by streptozotocin. The administration of EAHP significantly reduces blood glucose levels in mice compared to negative control groups, indicating its potential as a treatment for diabetes. The pancreas histology examination of the group that received EAHP shows a better condition than the pancreas in the negative control group, indicating that EAHP can protect pancreatic tissue from further damage caused by necrosis due to STZ.

Conclusion

In conclusion, this study suggests that Puguntano Herbal Water Extract (EAHP) has the potential as a blood glucose-lowering agent and can contribute to prevent further damage to the pancreas tissue in mice with diabetes induced by streptozotocin. Further research needs to be done to explore the mechanism of action and effectiveness in the treatment of diabetes in humans. Puguntano can be a promising and affordable treatment for diabetes mellitus.

Recommendations

Based on the results of this study, the following recommendations are made:

  1. Further research: Further research needs to be done to explore the mechanism of action and effectiveness in the treatment of diabetes in humans.
  2. Clinical trials: Clinical trials should be conducted to evaluate the safety and efficacy of Puguntano Herbal Water Extract (EAHP) in humans.
  3. Standardization: The standardization of Puguntano Herbal Water Extract (EAHP) is necessary to ensure its quality and consistency.
  4. Combination therapy: The combination of Puguntano Herbal Water Extract (EAHP) with other antidiabetic agents may be beneficial in the treatment of diabetes.

Limitations

This study has some limitations, including:

  1. Small sample size: The sample size of this study is small, which may limit the generalizability of the results.
  2. Short duration: The duration of this study is short, which may not be sufficient to evaluate the long-term effects of Puguntano Herbal Water Extract (EAHP).
  3. Limited information: Limited information is available on the mechanism of action of Puguntano Herbal Water Extract (EAHP), which may limit its potential as a treatment for diabetes.

Future Directions

Future studies should focus on:

  1. Mechanism of action: The mechanism of action of Puguntano Herbal Water Extract (EAHP) should be explored in detail to understand its potential as a treatment for diabetes.
  2. Clinical trials: Clinical trials should be conducted to evaluate the safety and efficacy of Puguntano Herbal Water Extract (EAHP) in humans.
  3. Combination therapy: The combination of Puguntano Herbal Water Extract (EAHP) with other antidiabetic agents may be beneficial in the treatment of diabetes.
  4. Standardization: The standardization of Puguntano Herbal Water Extract (EAHP) is necessary to ensure its quality and consistency.
    Q&A: The Effect of Puguntano Herbal Water Extract (Picria Fel-Terrae Lour.) Against a Decrease in Blood Glucose Levels Induced by Streptozotocin

Q: What is Puguntano Herbal Water Extract (EAHP)?

A: Puguntano Herbal Water Extract (EAHP) is a natural extract obtained from the plant Puguntano (Picria Fel-Terrae Lour.), which has been traditionally used in folk medicine to treat various health conditions, including diabetes.

Q: What is the purpose of this study?

A: The purpose of this study is to evaluate the effect of Puguntano Herbal Water Extract (EAHP) on blood glucose levels in mice induced by streptozotocin (STZ), a chemical that is commonly used to induce diabetes in experimental animals.

Q: What were the results of the study?

A: The results of the study showed that the administration of Puguntano Herbal Water Extract (EAHP) significantly reduced blood glucose levels in mice compared to negative control groups. The most significant decrease in glucose levels occurred in groups that received a dose of 200 mg/kg of body weight.

Q: How does Puguntano Herbal Water Extract (EAHP) work?

A: The exact mechanism of action of Puguntano Herbal Water Extract (EAHP) is not fully understood, but it is believed to work by stimulating the release of insulin from the pancreas, improving insulin sensitivity, and reducing glucose production in the liver.

Q: Is Puguntano Herbal Water Extract (EAHP) safe?

A: Puguntano Herbal Water Extract (EAHP) is generally considered safe, but it may cause side effects such as nausea, vomiting, and diarrhea in some individuals. Further research is needed to fully understand the safety profile of Puguntano Herbal Water Extract (EAHP).

Q: Can Puguntano Herbal Water Extract (EAHP) be used as a treatment for diabetes?

A: While Puguntano Herbal Water Extract (EAHP) has shown promise in reducing blood glucose levels in mice, it is not yet clear whether it can be used as a treatment for diabetes in humans. Further research is needed to fully understand its potential as a treatment for diabetes.

Q: What are the limitations of this study?

A: The limitations of this study include a small sample size, short duration, and limited information on the mechanism of action of Puguntano Herbal Water Extract (EAHP).

Q: What are the future directions for research on Puguntano Herbal Water Extract (EAHP)?

A: Future studies should focus on exploring the mechanism of action of Puguntano Herbal Water Extract (EAHP), conducting clinical trials to evaluate its safety and efficacy in humans, and investigating its potential as a combination therapy with other antidiabetic agents.

Q: Can Puguntano Herbal Water Extract (EAHP) be used in combination with other treatments for diabetes?

A: Yes, Puguntano Herbal Water Extract (EAHP) may be used in combination with other treatments for diabetes, such as metformin, sulfonylureas, and insulin. However, further research is needed to fully understand its potential as a combination therapy.

Q: How can I obtain Puguntano Herbal Water Extract (EAHP)?

A: Puguntano Herbal Water Extract (EAHP) is not yet commercially available, but it may be obtained from some herbal supplement manufacturers or online retailers. However, it is essential to consult with a healthcare professional before using any herbal supplement, especially if you have a pre-existing medical condition or are taking medications.

Q: What are the potential benefits of using Puguntano Herbal Water Extract (EAHP) as a treatment for diabetes?

A: The potential benefits of using Puguntano Herbal Water Extract (EAHP) as a treatment for diabetes include:

  • Reduced blood glucose levels
  • Improved insulin sensitivity
  • Reduced glucose production in the liver
  • Potential as a combination therapy with other antidiabetic agents
  • Natural and non-invasive treatment option

Q: What are the potential risks of using Puguntano Herbal Water Extract (EAHP) as a treatment for diabetes?

A: The potential risks of using Puguntano Herbal Water Extract (EAHP) as a treatment for diabetes include:

  • Side effects such as nausea, vomiting, and diarrhea
  • Interactions with other medications
  • Unknown long-term effects
  • Potential for over-supplementation

Q: How can I stay up-to-date with the latest research on Puguntano Herbal Water Extract (EAHP)?

A: You can stay up-to-date with the latest research on Puguntano Herbal Water Extract (EAHP) by:

  • Following reputable scientific journals and online resources
  • Attending conferences and workshops on herbal medicine and diabetes
  • Consulting with a healthcare professional or registered dietitian
  • Participating in online forums and discussion groups on herbal medicine and diabetes