The Effect Of Hydrolysis Time On Glucose Levels Of Α Cellulose Of Sugarcane (saccharum Officanarum L) Uses 15% HCl And Cellulase Enzymes Isolated From The Golden Snail Pancreas (pomacea Caniculata)

Feb 27, 2025 by ADMIN 198 views

The Effect of Hydrolysis Time on Glucose Levels of α Cellulose of Sugarcane (Saccharum officinarum L) Using 15% HCl and Cellulase Enzymes Isolated from the Golden Snail Pancreas (Pomacea caniculata)

Introduction

Understanding the Importance of Hydrolysis Time in Glucose Production

The production of glucose from cellulose is a crucial step in various industrial applications, including bioenergy and bioprocess production. Sugarcane (Saccharum officinarum L) is a potential source of glucose, and its pulp contains α cellulose, which can be converted into glucose through hydrolysis. Hydrolysis time is a critical factor that affects the glucose levels produced from α cellulose. In this study, we investigated the effect of hydrolysis time on glucose levels using 15% HCl and cellulase enzymes isolated from the golden snail pancreas (Pomacea caniculata).

Research Methodology

Isolation and Purification of Cellulase Enzymes

The cellulase enzymes were isolated from the golden snail pancreas and purified through the depositional process using ammonium sulfate with varying concentrations (0-20%, 20-40%, 40-60%, and 60-80% (B/V)). The activity of the cellulase enzyme produced was measured using the Nelson Somogyi method.

Hydrolysis Process

The cellulase enzymes with the highest activity were applied to the hydrolysis process of α cellulose with 15% HCl at varying times (90 minutes, 120 minutes, and 150 minutes). The glucose levels produced from hydrolysis were measured using the same method, Nelson Somogyi.

Results

Glucose Levels Produced from Hydrolysis

The measurement results of glucose levels show that glucose concentration increases at 120 minutes with a level of 0.0431 mg/ml. Meanwhile, glucose levels at 90 minutes and 150 minutes are 0.0359 mg/ml and 0.0327 mg/ml each.

Comparison of Glucose Levels Produced by HCl and Cellulase Enzymes

The results indicate that the longer hydrolysis time with 15% HCl tends to give better results in terms of glucose levels compared to the use of cellulase enzymes in the same time span.

Analysis of Results

The Effect of Hydrolysis Time on Glucose Levels

The results of this study indicate that the hydrolysis time has a significant effect on the glucose levels produced. Increased glucose levels in 120 minutes show that sufficient time for the hydrolysis process is very important in achieving optimal results.

Comparison of HCl and Cellulase Enzymes

The difference in glucose levels produced by HCl and cellulase enzymes can also be attributed to the different mechanisms of work of the two methods. HCL acts as a strong hydrolysis agent, able to break down β-glycosidic bonds in cellulose more effectively than enzymes, which require certain conditions to function optimally.

Conclusion

The Significance of Hydrolysis Time in Glucose Production

Overall, this study succeeded in showing that the hydrolysis time has a significant effect on the glucose levels of the α cellulose of sugarcane pulp using 15% HCl and cellulase enzymes. This finding provides valuable information in the development of the process of utilizing sugarcane pulp as a potential source of glucose, which can be used in various industrial applications, including bioenergy and other bioprocess production.

The Importance of Sustainable Biomass Processing

With a better understanding of this optimal hydrolysis condition, it is expected to be obtained an increase in efficiency in the conversion of biomass resources into useful products. The use of cellulase enzymes is more environmentally friendly and has the potential to be a more sustainable choice in biomass processing.

Future Directions

Further Research on Hydrolysis Time and Enzyme Activity

Further research is needed to investigate the effect of hydrolysis time on enzyme activity and glucose levels produced from α cellulose. This study provides a foundation for the development of more efficient and sustainable biomass processing methods.

The Potential of Sugarcane Pulp as a Source of Glucose

The results of this study highlight the potential of sugarcane pulp as a source of glucose. Further research is needed to explore the feasibility of using sugarcane pulp as a feedstock for bioenergy and bioprocess production.

References

Nelson, N., & Somogyi, M. (1926). A method for the determination of glucose in blood and urine. Journal of Biological Chemistry, 78(2), 443-454.
Pomacea caniculata. (n.d.). In Wikipedia. Retrieved from https://en.wikipedia.org/wiki/Pomacea_caniculata
Saccharum officinarum L. (n.d.). In Wikipedia. Retrieved from https://en.wikipedia.org/wiki/Saccharum_officinarum
Frequently Asked Questions (FAQs) about the Effect of Hydrolysis Time on Glucose Levels of α Cellulose of Sugarcane (Saccharum officinarum L)

Q: What is the purpose of this study?

A: The purpose of this study is to investigate the effect of hydrolysis time on glucose levels produced from α cellulose of sugarcane pulp using 15% HCl and cellulase enzymes isolated from the golden snail pancreas (Pomacea caniculata).

Q: What is the significance of hydrolysis time in glucose production?

A: Hydrolysis time is a critical factor that affects the glucose levels produced from α cellulose. The results of this study show that the longer hydrolysis time with 15% HCl tends to give better results in terms of glucose levels compared to the use of cellulase enzymes in the same time span.

Q: What are the differences between HCl and cellulase enzymes in glucose production?

A: HCl acts as a strong hydrolysis agent, able to break down β-glycosidic bonds in cellulose more effectively than enzymes, which require certain conditions to function optimally. The use of cellulase enzymes is more environmentally friendly and has the potential to be a more sustainable choice in biomass processing.

Q: What are the potential applications of this study?

A: The results of this study provide valuable information in the development of the process of utilizing sugarcane pulp as a potential source of glucose, which can be used in various industrial applications, including bioenergy and other bioprocess production.

Q: What are the limitations of this study?

A: This study has several limitations, including the use of a single type of enzyme and the limited range of hydrolysis times tested. Further research is needed to investigate the effect of hydrolysis time on enzyme activity and glucose levels produced from α cellulose.

Q: What are the future directions of this research?

A: Further research is needed to investigate the effect of hydrolysis time on enzyme activity and glucose levels produced from α cellulose. This study provides a foundation for the development of more efficient and sustainable biomass processing methods.

Q: What are the potential benefits of using sugarcane pulp as a source of glucose?

Q: What are the potential environmental benefits of using cellulase enzymes?

A: The use of cellulase enzymes is more environmentally friendly and has the potential to be a more sustainable choice in biomass processing. This is because cellulase enzymes can be produced through fermentation, which is a more environmentally friendly process compared to the production of HCl.

Q: What are the potential economic benefits of using sugarcane pulp as a source of glucose?

A: The results of this study highlight the potential of sugarcane pulp as a source of glucose. Further research is needed to explore the feasibility of using sugarcane pulp as a feedstock for bioenergy and bioprocess production, which could lead to economic benefits for the industry.

Q: What are the potential social benefits of using sugarcane pulp as a source of glucose?

Q: What are the potential future applications of this research?

A: The results of this study provide a foundation for the development of more efficient and sustainable biomass processing methods. Further research is needed to explore the potential applications of this research, including the use of sugarcane pulp as a feedstock for bioenergy and bioprocess production.