The Effect Of Giving Benzyl Amino Purine (BAP) And Naphthalene Acetic Acid (NAA) In Induction Of Temulawak Plant Shoots (curcuma Xanthorrhiza Roxb)

Feb 27, 2025 by ADMIN 148 views

The Effect of Giving Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) in Induction of Temulawak Plant Shoots (Curcuma Xanthorrhiza Roxb)

Medicinal plants with rhizome types, such as ginger (Curcuma xanthorrhiza Roxb), have a very important role as a source of drugs, both at the local and global levels. The cultivation of these plants is a significant challenge, as the amount of seed produced tends to be small, and the time needed for the seed seeding process is relatively long. This causes the availability of seeds to be insufficient. In vitro culture techniques emerged as a promising alternative method to increase crop production commercially. This study aims to determine the effect of giving Benzyl Amino Purine (BAP) and Naphthalene acetic Acid (NAA) on Murashige and SKOOG (MS) media in inducing ginger explants through vitro culture.

Background and Significance of the Study

Ginger (Curcuma xanthorrhiza Roxb) is a medicinal plant that has been widely used for its medicinal properties. However, the cultivation of this plant is facing several challenges, including the limited availability of seeds and the long time required for seed germination. In vitro culture techniques have been developed as a promising alternative method to increase crop production commercially. This study aims to investigate the effect of giving BAP and NAA on MS media in inducing ginger explants through vitro culture.

Methodology

The study was conducted in the Alifa Agricultural Research Center (Alifa-Arc) Center Culture Laboratory located in Medan, North Sumatra, from June to October 2022. In this study, a random design was used complete with two treatment factors, namely the concentration of BAP (0 mg/L, 2 mg/L, 4 mg/L, and 6 mg/L) and NAA concentration (0 mg/L, 0.5 mg/L, and 1 mg/L). The results showed that the combination of BAP and NAA had a significant influence on the number of shoots and bud lengths, but had no significant effect on the number of roots and root length. The highest concentration that gives optimal results in the form of the number of shoots and the length of the shoots is achieved in the treatment of 6 mg/L BAP with 0.5 mg/L NAA.

Analysis of Research Results

In the context of this research, the combination of BAP and NAA is very important to improve the process of propagating ginger plants. BAP, as a cytokinin hormone, plays a role in stimulating cell division and shoot formation, while NAA, which is included in the auxin group, functions in the formation of roots. The use of the combination of these two hormones appropriately can maximize the growth of explants, encourage the formation of more and longer shoots.

It is important to remember that each type of plant has a different response to the type and concentration of hormones given. In this study, a higher BAP concentration gives better results compared to NAA concentration. This shows that for ginger, the trigger for shoots is more dominant than the formation of roots in vitro culture conditions. This may be caused by the nature of ginger which tends to produce more shoots than roots in normal growing conditions.

Implications of the Study

With the increasing needs of medicinal plants, in vitro culture techniques can be an efficient solution to increase the availability of ginger seeds. Through appropriate hormone adjustments, farmers and researchers can accelerate the process of reproduction of this plant, which in turn can meet the increasing market needs. Further research needs to be carried out to explore more deeply about the effect of concentration and other hormone combinations, as well as optimal environmental conditions for the multiplication of in vitro.

Conclusion

In conclusion, this study has shown that the combination of BAP and NAA has a significant influence on the number of shoots and bud lengths, but has no significant effect on the number of roots and root length. The highest concentration that gives optimal results in the form of the number of shoots and the length of the shoots is achieved in the treatment of 6 mg/L BAP with 0.5 mg/L NAA. This study has implications for the cultivation of ginger plants, as it provides a promising alternative method to increase crop production commercially. Further research needs to be carried out to explore more deeply about the effect of concentration and other hormone combinations, as well as optimal environmental conditions for the multiplication of in vitro.

Recommendations

Based on the findings of this study, the following recommendations are made:

Further research needs to be carried out to explore more deeply about the effect of concentration and other hormone combinations on the multiplication of ginger plants in vitro.
Optimal environmental conditions for the multiplication of in vitro need to be explored to improve the efficiency of the process.
The use of BAP and NAA in combination with other hormones needs to be investigated to improve the growth and development of ginger plants in vitro.

Limitations of the Study

This study has several limitations, including:

The study was conducted in a controlled environment, and the results may not be applicable to field conditions.
The study only investigated the effect of BAP and NAA on the multiplication of ginger plants in vitro, and did not explore other factors that may influence the process.
The study only used a limited number of concentrations of BAP and NAA, and did not explore other concentrations that may be effective.

Future Research Directions

Based on the findings of this study, the following future research directions are suggested:

Investigating the effect of other hormones on the multiplication of ginger plants in vitro.
Exploring optimal environmental conditions for the multiplication of in vitro.
Investigating the use of BAP and NAA in combination with other hormones to improve the growth and development of ginger plants in vitro.

Conclusion

Q: What is the purpose of this study? A: The purpose of this study is to determine the effect of giving Benzyl Amino Purine (BAP) and Naphthalene acetic Acid (NAA) on Murashige and SKOOG (MS) media in inducing ginger explants through vitro culture.

Q: What are the benefits of using BAP and NAA in vitro culture? A: The use of BAP and NAA in vitro culture can maximize the growth of explants, encourage the formation of more and longer shoots, and improve the efficiency of the process.

Q: What are the limitations of this study? A: This study has several limitations, including the controlled environment, the limited number of concentrations of BAP and NAA, and the lack of exploration of other factors that may influence the process.

Q: What are the implications of this study for the cultivation of ginger plants? A: This study provides a promising alternative method to increase crop production commercially, and can meet the increasing market needs of medicinal plants.

Q: What are the future research directions suggested by this study? A: The future research directions suggested by this study include investigating the effect of other hormones on the multiplication of ginger plants in vitro, exploring optimal environmental conditions for the multiplication of in vitro, and investigating the use of BAP and NAA in combination with other hormones to improve the growth and development of ginger plants in vitro.

Q: What are the potential applications of this study? A: The potential applications of this study include the commercial production of ginger plants, the development of new medicinal products, and the improvement of crop yields.

Q: What are the potential risks associated with the use of BAP and NAA in vitro culture? A: The potential risks associated with the use of BAP and NAA in vitro culture include the possibility of contamination, the risk of over-production of shoots, and the potential for genetic modification of the plant.

Q: How can the results of this study be applied in practice? A: The results of this study can be applied in practice by using BAP and NAA in vitro culture to improve the efficiency of the process, to increase crop yields, and to develop new medicinal products.

Q: What are the next steps in this research? A: The next steps in this research include further investigation of the effect of concentration and other hormone combinations, as well as optimal environmental conditions for the multiplication of in vitro.

Q: How can the results of this study be used to improve the cultivation of ginger plants? A: The results of this study can be used to improve the cultivation of ginger plants by using BAP and NAA in vitro culture to improve the efficiency of the process, to increase crop yields, and to develop new medicinal products.

Q: What are the potential benefits of using BAP and NAA in vitro culture for the cultivation of ginger plants? A: The potential benefits of using BAP and NAA in vitro culture for the cultivation of ginger plants include improved efficiency, increased crop yields, and the development of new medicinal products.

Q: How can the results of this study be used to improve the production of ginger plants? A: The results of this study can be used to improve the production of ginger plants by using BAP and NAA in vitro culture to improve the efficiency of the process, to increase crop yields, and to develop new medicinal products.

Q: What are the potential applications of this study for the production of ginger plants? A: The potential applications of this study for the production of ginger plants include the commercial production of ginger plants, the development of new medicinal products, and the improvement of crop yields.