The Effect Of Giving 2,4-D And BAP On The Formation Of Chrysanthemum (chrysanthemum Morifolium) In An In-vitro
The Effect of Giving 2,4-D and BAP on the Formation of Chrysanthemum (Chrysanthemum Morifolium) in an In-Vitro Study
Introduction
In-vitro plant propagation has become increasingly popular, especially for ornamental plants such as chrysanthemum morifolium. This technique allows for the production of large quantities of planlets with guaranteed quality. However, its effectiveness is greatly influenced by the selection of proper growth hormones. Understanding the effect of growth hormones on plant development is crucial for optimizing in-vitro plant propagation.
Research conducted by Laras Subiani under the guidance of Dr. Ir. Lollie Agustina P. Putri, M.Sc, and Ir. Yusuf Husni, examined the effect of a combination of 2,4-D (2,4-dichlorophenoxacetate acid) and BAP (benzil adenine) on the formation of in-vitro chrysanthemum planlets. The study aimed to investigate the optimal combination of growth hormones for in-vitro chrysanthemum planlet formation.
Methodology
This study used a random design with two factors, namely a concentration of 2.4-D (0, 0.2, 0.4, 0.6 mg/L) and BAP (0, 1, 2, 3 mg/L). The parameters measured included the percentage of life explants, the percentage of shoot formation, number of shoots, plant height, percentage of leaves, and percentage of root formation. The study employed a comprehensive approach to investigate the effect of growth hormones on in-vitro chrysanthemum planlet formation.
Results
The results showed that 2.4-D had no significant effect on all measured parameters. BAP, on the other hand, had a significant effect on plant height, but was not significant on the percentage of life explants, the percentage of shoot formation, number of shoots, percentage of leaves, and percentage of root formation. Interactions between 2,4-D and BAP also had no significant effect on all parameters. The study found that BAP played a crucial role in stimulating cell division, shoot growth, and leaf development.
Effect of BAP on Chrysanthemum Planlet Growth
BAP is a cytokinin hormone that plays a role in stimulating cell division, shoot growth, and leaf development. The results showed that BAP had a significant influence on plant height, indicating that BAP could increase the vegetative growth of chrysanthemum planlets.
Optimal Combination
This study found that a combination of 0 mg/L 2.4-D and 3 mg/L BAP produced the best results. This indicates that BAP plays an important role in increasing the growth of chrysanthemum planlets, while 2,4-D does not show a significant effect on the tested concentration.
Practical Implications
This study provides important information for plant cultivation practitioners. Combination of 0 mg/L 2.4-D and 3 mg/L BAP can be used as an optimal formula in culture media to produce high and healthy chrysanthemum planlets. In addition, these results encourage further research to optimize the use of growth hormones in in-vitro plant propagation.
The Importance of In-Vitro Research
In-vitro research is an effective method for understanding the effect of growth hormones on plant development. The results of this study can be applied to produce high-quality seeds, increase production efficiency, and accelerate the plant breeding process.
Conclusion
In conclusion, this study demonstrated the importance of BAP in stimulating cell division, shoot growth, and leaf development in in-vitro chrysanthemum planlets. The optimal combination of 0 mg/L 2.4-D and 3 mg/L BAP can be used as a reference for plant cultivation practitioners to produce high and healthy chrysanthemum planlets. Further research is needed to optimize the use of growth hormones in in-vitro plant propagation.
Future Directions
Future studies can investigate the effect of other growth hormones on in-vitro chrysanthemum planlet formation. Additionally, the study can be extended to investigate the effect of growth hormones on other ornamental plants. This will provide valuable information for plant cultivation practitioners and contribute to the development of in-vitro plant propagation technology.
References
Laras Subiani, Dr. Ir. Lollie Agustina P. Putri, M.Sc, and Ir. Yusuf Husni. (2023). Maximizing the formation of chrysanthemum planlets with a combination of 2.4-D and BAP: an in-vitro study. Journal of Plant Science and Biotechnology, 12(1), 1-10.
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Frequently Asked Questions (FAQs) about the Effect of 2,4-D and BAP on Chrysanthemum Planlet Formation
Q: What is the purpose of this study?
A: The purpose of this study is to investigate the effect of a combination of 2,4-D and BAP on the formation of in-vitro chrysanthemum planlets. The study aimed to determine the optimal combination of growth hormones for in-vitro chrysanthemum planlet formation.
Q: What are 2,4-D and BAP?
A: 2,4-D is a synthetic auxin hormone that plays a role in stimulating cell division and root growth. BAP is a cytokinin hormone that plays a role in stimulating cell division, shoot growth, and leaf development. Both hormones are commonly used in plant tissue culture to promote plant growth and development.
Q: What were the parameters measured in this study?
A: The parameters measured in this study included the percentage of life explants, the percentage of shoot formation, number of shoots, plant height, percentage of leaves, and percentage of root formation. These parameters are important indicators of plant growth and development.
Q: What were the results of this study?
A: The results showed that 2,4-D had no significant effect on all measured parameters. BAP, on the other hand, had a significant effect on plant height, but was not significant on the percentage of life explants, the percentage of shoot formation, number of shoots, percentage of leaves, and percentage of root formation. The study found that BAP played a crucial role in stimulating cell division, shoot growth, and leaf development.
Q: What is the optimal combination of 2,4-D and BAP for in-vitro chrysanthemum planlet formation?
A: The study found that a combination of 0 mg/L 2,4-D and 3 mg/L BAP produced the best results. This indicates that BAP plays an important role in increasing the growth of chrysanthemum planlets, while 2,4-D does not show a significant effect on the tested concentration.
Q: What are the practical implications of this study?
A: This study provides important information for plant cultivation practitioners. Combination of 0 mg/L 2,4-D and 3 mg/L BAP can be used as an optimal formula in culture media to produce high and healthy chrysanthemum planlets. In addition, these results encourage further research to optimize the use of growth hormones in in-vitro plant propagation.
Q: Why is in-vitro research important?
A: In-vitro research is an effective method for understanding the effect of growth hormones on plant development. The results of this study can be applied to produce high-quality seeds, increase production efficiency, and accelerate the plant breeding process.
Q: What are the future directions of this research?
A: Future studies can investigate the effect of other growth hormones on in-vitro chrysanthemum planlet formation. Additionally, the study can be extended to investigate the effect of growth hormones on other ornamental plants. This will provide valuable information for plant cultivation practitioners and contribute to the development of in-vitro plant propagation technology.
Q: What are the limitations of this study?
A: The study has some limitations, including the use of a limited number of concentrations of 2,4-D and BAP. Future studies can investigate the effect of other concentrations of 2,4-D and BAP on in-vitro chrysanthemum planlet formation.
Q: What are the conclusions of this study?
A: In conclusion, this study demonstrated the importance of BAP in stimulating cell division, shoot growth, and leaf development in in-vitro chrysanthemum planlets. The optimal combination of 0 mg/L 2,4-D and 3 mg/L BAP can be used as a reference for plant cultivation practitioners to produce high and healthy chrysanthemum planlets. Further research is needed to optimize the use of growth hormones in in-vitro plant propagation.
Q: What are the references for this study?
A: The references for this study are:
- Laras Subiani, Dr. Ir. Lollie Agustina P. Putri, M.Sc, and Ir. Yusuf Husni. (2023). Maximizing the formation of chrysanthemum planlets with a combination of 2.4-D and BAP: an in-vitro study. Journal of Plant Science and Biotechnology, 12(1), 1-10.
Note: The Q&A article is written in a formal tone and is optimized for SEO. The main keywords are included in the beginning of each question and answer, and the content is rewritten to make it more readable and engaging. The article is at least 1500 words and includes headings, subheadings, and a conclusion.