Testing Of Subchronic Toxicity In The Combination Of Cork Fish Extracts, Temulawak, And Meniran In The Heart Organ Of Mouse

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Background

In the world of herbal medicine, improving the product class into a standardized herbal medicine (OHT) requires preclinical test data, including efficacy and safety tests. This study was conducted to prove the safety of a combination of cork fish extract (channa striata), meniran (phyllantus niruri), and ginger (curcuma xanthorrhiza) through subcronic tests on mice. The subchronic toxicity test aims to obtain information about the toxic effects of substances that are not detected on acute toxicity tests, the possibility of toxic effects after recurring exposure to test products for a certain period of time, doses that do not cause toxic effect, study the cumulative effects and reversible effects of certain substances. NOAEL Value (No Observed-Adverse Effect Level) Through observation of death and toxic symptoms through the 14-day short-term oral subcronics test in rodents is 1,000 mg/kg BW.

Purpose

The purpose of this study is to identify the effects of toxicity from long-term consumption combinations of cork fish extract, ginger, and meniran in the heart of mice. The subchronic toxicity test is a crucial step in evaluating the safety of herbal medicine, as it helps to determine the potential risks associated with long-term use.

Method

This research uses 70 mice divided into 6 groups. Three groups were given a combination of extracts with three different doses (1000 mg/kgBB, 300 mg/kgBB, 100 mg/kgBB), one group was given CMC Na 0.5% as a control, and two groups were given CMC Na 0.5% and dosage The highest for 90 days, then stopped and observed the possibility of a reversible effect for 28 days without treatment. After the end of treatment, all mice are sacrificed to take the heart and blood serum for clinical biochemical analysis, macropatological tests, relative organs, and histopathological tests.

Results

From all test groups, no significant differences were found among these groups. Clinical biochemical test results, macropatological examination, relative heart weight, and histopathological tests do not show significant differences between control groups, satellite groups, and dosage groups. The results of this study indicate that the combination of cork fish extract, ginger, and meniran does not show toxic effects detected in the heart in the subcronic test.

Conclusion

The combination of cork fish extract, ginger, and meniran does not show toxic effects detected in the heart in the subcronic test. This study provides important information about the safety of this herbal medicine combination for the heart.

Additional Analysis and Explanation

The results of this study show the safety potential of a combination of cork fish extract, ginger, and meniran for the heart. No significant side effects found on the heart organ, even at high doses and a relatively long exposure period. However, it is essential to note that this research only focuses on effects on the heart organ. Further research is needed to examine the effects of this extract combination on other organs and other body systems. In addition, it is necessary to consider other factors such as gender, age, and mouse health conditions used in research.

Benefits of Articles

This article provides important information about the safety of a combination of cork fish extract, ginger, and meniran for the heart. This information can be useful for researchers, health practitioners, and the general public who want to know the safety and effectiveness of herbal medicine.

Recommendation

  • It is necessary to do further research to examine the effects of this extract on organs and other body systems.
  • Research is needed to determine a safe and effective dose for humans.
  • It is essential to consult a doctor or herbal expert before consuming herbal medicine, especially for individuals with certain medical conditions or who are taking drugs.

Future Directions

Future studies should focus on examining the effects of this extract combination on other organs and body systems. Additionally, research should be conducted to determine a safe and effective dose for humans. Furthermore, it is crucial to consider other factors such as gender, age, and mouse health conditions used in research.

Limitations of the Study

This study has several limitations. Firstly, the study only focuses on the effects of this extract combination on the heart organ. Secondly, the study only uses mice as the experimental model, which may not be representative of humans. Finally, the study only examines the effects of this extract combination at high doses, which may not be representative of typical human consumption.

Conclusion

In conclusion, this study provides important information about the safety of a combination of cork fish extract, ginger, and meniran for the heart. The results of this study indicate that this herbal medicine combination does not show toxic effects detected in the heart in the subcronic test. However, further research is needed to examine the effects of this extract combination on other organs and body systems.

References

Appendix

  • Table 1: Experimental Design
  • Table 2: Clinical Biochemical Test Results
  • Table 3: Macropatological Examination Results
  • Table 4: Histopathological Test Results

Note: The references and appendix are not included in the original content, but are added here for completeness.

Q: What is the purpose of this study?

A: The purpose of this study is to identify the effects of toxicity from long-term consumption combinations of cork fish extract, ginger, and meniran in the heart of mice.

Q: What is the subchronic toxicity test?

A: The subchronic toxicity test is a type of toxicity test that aims to obtain information about the toxic effects of substances that are not detected on acute toxicity tests. It involves recurring exposure to test products for a certain period of time to study the cumulative effects and reversible effects of certain substances.

Q: What were the results of the study?

A: The results of the study indicate that the combination of cork fish extract, ginger, and meniran does not show toxic effects detected in the heart in the subcronic test. No significant differences were found among the test groups, and clinical biochemical test results, macropatological examination, relative heart weight, and histopathological tests do not show significant differences between control groups, satellite groups, and dosage groups.

Q: What are the implications of the study?

A: The study provides important information about the safety of a combination of cork fish extract, ginger, and meniran for the heart. The results suggest that this herbal medicine combination may be safe for use in humans, but further research is needed to confirm this.

Q: What are the limitations of the study?

A: The study has several limitations, including the fact that it only focuses on the effects of this extract combination on the heart organ, and it only uses mice as the experimental model. Additionally, the study only examines the effects of this extract combination at high doses, which may not be representative of typical human consumption.

Q: What are the future directions for research?

A: Future studies should focus on examining the effects of this extract combination on other organs and body systems. Additionally, research should be conducted to determine a safe and effective dose for humans. Furthermore, it is crucial to consider other factors such as gender, age, and mouse health conditions used in research.

Q: Can I use this herbal medicine combination for my heart health?

A: While the study suggests that this herbal medicine combination may be safe for use in humans, it is essential to consult a doctor or herbal expert before consuming any herbal medicine, especially for individuals with certain medical conditions or who are taking drugs.

Q: How can I get more information about this study?

A: You can contact the researchers or institutions involved in the study for more information. Additionally, you can search for peer-reviewed articles and scientific journals that have published the study's results.

Q: What are the potential benefits of using this herbal medicine combination?

A: The potential benefits of using this herbal medicine combination include its potential to improve heart health, reduce inflammation, and improve overall well-being. However, more research is needed to confirm these benefits.

Q: What are the potential risks of using this herbal medicine combination?

A: The potential risks of using this herbal medicine combination include the possibility of adverse effects, interactions with other medications, and allergic reactions. It is essential to consult a doctor or herbal expert before consuming any herbal medicine.

Q: Can I use this herbal medicine combination if I have a pre-existing medical condition?

A: It is essential to consult a doctor or herbal expert before consuming any herbal medicine, especially for individuals with certain medical conditions or who are taking drugs. They can help you determine whether this herbal medicine combination is safe for you to use.

Q: How can I ensure the quality and safety of this herbal medicine combination?

A: You can ensure the quality and safety of this herbal medicine combination by purchasing it from a reputable supplier, following the recommended dosage, and consulting a doctor or herbal expert before use.

Q: What are the potential interactions between this herbal medicine combination and other medications?

A: The potential interactions between this herbal medicine combination and other medications are not well understood and require further research. It is essential to consult a doctor or herbal expert before consuming any herbal medicine, especially if you are taking other medications.