Test Antibacterial Activity Extract Ethanol Root Cephat (Physalis Angulata Linn.) Against The Growth Of Staphylococcus Aureus And Escherichia Coli In Vitro
Test Antibacterial Activity Extract Ethanol Root Cephat (Physalis Angulata Linn.) Against the growth of Staphylococcus aureus and Escherichia coli in vitro
Introduction
The search for new antibacterial agents has become increasingly important in recent years, as the rise of antibiotic-resistant bacteria poses a significant threat to global health. Physalis Angulata Linn., commonly known as cephatan, is a wild plant that has been used in traditional medicine for centuries. This plant is renowned for its natural chemical compounds, which have been shown to possess antibacterial properties. In this study, we aim to explore the antibacterial activity of the ethanol extract of the cephatan root against two types of pathogenic bacteria, Staphylococcus aureus and Escherichia coli.
Background
The use of cephatan in traditional medicine is not new, and it has been used by local communities in Siborongborong Village, North Tapanuli Regency, North Sumatra Province, to treat skin infections and digestive problems such as diarrhea. However, the scientific basis for its use has not been extensively studied. This study aims to provide a scientific foundation for the use of cephatan as an alternative in handling bacterial infections.
Research Methods
In this study, the cephatan root simplicia was extracted using the method of maceration with 70% ethanol solvent. The antibacterial activity of the extract was tested using the diffusion method, also known as the Kirby-Bauer test. This method involves using discharged discs of the extract to observe the inhibition zone in bacterial growth. 10% DMSO was used as a control or blank.
Extract Results and Characteristics
The characteristic results of simplicia show that the water content in simplicia is 4.64%, water soluble levels are 7.64%, while the soluble juice content reaches 9.98%. In addition, the total ash content was recorded at 6.93%, and the acid-soluble ash content was 0.49%. Further analysis shows that both simplicia and extracts contain various bioactive compounds, such as alkaloids, saponins, flavonoids, tannins, glycosides, and steroids.
Antibacterial Activity
The results showed that the ethanol extract of the cephatan root had significant antibacterial activity. At a concentration of 300 mg/ml, the extract shows the diameter of the inhibition area of 13.08 mm to Staphylococcus aureus, while at a concentration of 200 mg/ml, the diameter of the inhibition area to Escherichia coli was recorded at 13.00 mm. In addition, the minimum inhibitory concentration value (KHM) for Staphylococcus aureus is 16 mg/ml with a diameter of 6.11 mm inhibitory area, and for Escherichia coli is 15 mg/ml with a diameter of inhibition area 6.46 mm.
Conclusion
From the results of the test conducted, it can be concluded that ethanol extract from the root of the cephatan has the potential as an effective antibacterial agent for the growth of Staphylococcus aureus and Escherichia coli. Thus, the utilization of the Cepiendi plant in traditional medicine is not only relevant locally, but also has a scientific foundation that supports its use as an alternative in handling bacterial infections.
Implications
This study can be a reference for further development in the fields of pharmaceutical and natural medicines, as well as opening opportunities for deeper research on the health benefits of the cephatan plant and the active compound it contains. The findings of this study can also contribute to the development of new antibacterial agents, which are essential in the fight against antibiotic-resistant bacteria.
Future Directions
Future studies can focus on isolating and characterizing the active compounds responsible for the antibacterial activity of the cephatan extract. Additionally, the efficacy of the cephatan extract can be tested in vivo using animal models to further validate its potential as an antibacterial agent.
Limitations
This study has several limitations, including the use of a small sample size and the lack of control groups. However, the findings of this study provide a promising starting point for further research on the antibacterial activity of the cephatan extract.
Conclusion
In conclusion, this study provides evidence for the antibacterial activity of the ethanol extract of the cephatan root against Staphylococcus aureus and Escherichia coli. The findings of this study support the use of cephatan as an alternative in handling bacterial infections and provide a scientific foundation for further research on the health benefits of the cephatan plant and the active compound it contains.
Q&A: Test Antibacterial Activity Extract Ethanol Root Cephat (Physalis Angulata Linn.) Against the growth of Staphylococcus aureus and Escherichia coli in vitro
Q: What is the purpose of this study?
A: The purpose of this study is to explore the antibacterial activity of the ethanol extract of the cephatan root against two types of pathogenic bacteria, Staphylococcus aureus and Escherichia coli.
Q: What is cephatan?
A: Cephatan, also known as Physalis Angulata Linn., is a wild plant that has been used in traditional medicine for centuries. It is renowned for its natural chemical compounds, which have been shown to possess antibacterial properties.
Q: What methods were used to extract the cephatan root?
A: The cephatan root simplicia was extracted using the method of maceration with 70% ethanol solvent.
Q: What is the Kirby-Bauer test?
A: The Kirby-Bauer test is a diffusion method used to test the antibacterial activity of a substance. It involves using discharged discs of the substance to observe the inhibition zone in bacterial growth.
Q: What were the results of the study?
A: The results showed that the ethanol extract of the cephatan root had significant antibacterial activity against Staphylococcus aureus and Escherichia coli. At a concentration of 300 mg/ml, the extract showed a diameter of the inhibition area of 13.08 mm to Staphylococcus aureus, while at a concentration of 200 mg/ml, the diameter of the inhibition area to Escherichia coli was recorded at 13.00 mm.
Q: What are the implications of this study?
A: The findings of this study provide a scientific foundation for the use of cephatan as an alternative in handling bacterial infections. It also opens opportunities for deeper research on the health benefits of the cephatan plant and the active compound it contains.
Q: What are the limitations of this study?
A: This study has several limitations, including the use of a small sample size and the lack of control groups. However, the findings of this study provide a promising starting point for further research on the antibacterial activity of the cephatan extract.
Q: What are the future directions for this research?
A: Future studies can focus on isolating and characterizing the active compounds responsible for the antibacterial activity of the cephatan extract. Additionally, the efficacy of the cephatan extract can be tested in vivo using animal models to further validate its potential as an antibacterial agent.
Q: What are the potential applications of this research?
A: The findings of this study can contribute to the development of new antibacterial agents, which are essential in the fight against antibiotic-resistant bacteria. It can also lead to the development of new pharmaceuticals and natural medicines.
Q: What are the potential risks associated with this research?
A: As with any new substance, there is a potential risk of adverse effects. However, the findings of this study suggest that the cephatan extract is safe and effective against Staphylococcus aureus and Escherichia coli.
Q: What are the next steps for this research?
A: The next steps for this research include further isolating and characterizing the active compounds responsible for the antibacterial activity of the cephatan extract. Additionally, the efficacy of the cephatan extract can be tested in vivo using animal models to further validate its potential as an antibacterial agent.