Test Antibacterial Activity Combination Of Ethanol Extracts Sembung C Prophecy (Mikania Micrantha Kunth) And Guava Leaves (Psidium Guajava L.) Against Escherichia Coli And Bacillus Cereus

Feb 28, 2025 by ADMIN 188 views

Test Antibacterial Activity Combination of Ethanol Extracts Sembung C Prophecy (Mikania Micrantha Kunth) and Guava Leaves (Psidium Guajava L.) Against Escherichia coli and Bacillus cereus

Introduction

*Diarrhea is a major health problem in developing countries like Indonesia, which is often caused by bacteria such as *Escherichia coli *and *Bacillus cereus . The use of inappropriate antibiotics can trigger bacterial resistance, so the search for alternative treatments from natural ingredients is increasingly important.

In recent years, the search for natural antibiotics has gained significant attention due to the increasing problem of antibiotic resistance. Plants have been a rich source of bioactive compounds with antibacterial properties. Sembung vines (Mikania micrantha kunth) and guava leaves (psidium guajava L.) are known to have the potential as a source of natural antibiotics. This study aims to identify the content of phytochemicals in dry powder and ethanol extracts of vines and guava leaves, as well as to test the effectiveness of the combination of ethanol extract of both types of leaves to *Escherichia coli *and *Bacillus cereus *.

Background

Sembung vines (Mikania micrantha kunth) and guava leaves (psidium guajava L.) are known to have the potential as a source of natural antibiotics. Sembung vines contain bioactive compounds such as alkaloids, flavonoids, glycosides, tannins, and steroids/triterpenoids. Meanwhile, guava leaves contain flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids, which are known to have antibacterial activity.

The use of natural antibiotics is becoming increasingly important due to the increasing problem of antibiotic resistance. Plants have been a rich source of bioactive compounds with antibacterial properties. Sembung vines and guava leaves are known to have the potential as a source of natural antibiotics. This study aims to identify the content of phytochemicals in dry powder and ethanol extracts of vines and guava leaves, as well as to test the effectiveness of the combination of ethanol extract of both types of leaves to *Escherichia coli *and *Bacillus cereus *.

Research Methods

This study was conducted experimental, starting from the preparation of leaf dried powder, extraction using the maceration method, phytochemical screening test, dry powder and extracts, as well as testing antibacterial activity with the agar disk diffusion method. The combination of extracts was tested using the concentration of the Ethanol extract of 2 mg/ml of cembung leaves and ethanol extract of guava leaves with a concentration of 0.3 mg/ml; 0.6 mg/ml; 1.2 mg/ml. In addition, the Mic Ethanol Ethanol Extract of Guava Leaves 0.6 mg/ml is combined with an ethanol extract of the vines with a concentration of 1 mg/ml; 2 mg/ml; 4 mg/ml.

Research Result

Characterization of dried powder Sembung vines and guava leaves show a water content of 6.65% and 8.65%, water soluble extract content 25.98% and 21.49%, Ethanol soluble extract levels 12.19% and 20.51% , total ash content is 13.77% and 6.28%, and acid -soluble ash content 2.01% and 0.57%.

Phytochemical tests show that dry powder and ethanol extract of vines leaves contain alkaloids, flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids. Ethanol extract of guava leaves also contain flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids.

The results of testing the antibacterial activity of the combination of extracts on * Escherichia coli * and * Bacillus cereus * showed a larger resistance zone compared to each single extract. This shows that the combination of Ethanol extracts of creeping leaves and guava leaves gives a synergistic effect in inhibiting bacterial growth.

Conclusion

Combination of Ethanol Extract Sembung C proposal and guava leaves show higher antibacterial activity compared to each single extract. These results open up opportunities for the development of natural antibacterials from plants that are safe and effective in dealing with diarrhea due to bacterial infections.

Additional Analysis and Explanation

This study shows the potential combination of plant extracts in increasing antibacterial effectiveness. This can be caused by a synergistic effect between bioactive compounds in both plants. These compounds can work together to inhibit the growth of bacteria with various mechanisms, such as disrupting the formation of bacterial cell walls, inhibiting bacterial protein synthesis, or disrupting bacterial metabolism.

Further research needs to be carried out to identify bioactive compounds that play a role in the synergistic effect, determine the mechanism of action, and test the toxicity of the combination of extracts in animal models. The results of this study can be the basis for the development of natural antibacterial products that are safe and effective in dealing with diarrhea and other bacterial infections.

Future Directions

The results of this study can be the basis for the development of natural antibacterial products that are safe and effective in dealing with diarrhea and other bacterial infections. Further research needs to be carried out to identify bioactive compounds that play a role in the synergistic effect, determine the mechanism of action, and test the toxicity of the combination of extracts in animal models.

Conclusion

References

[1] Sembung vines (Mikania micrantha kunth) and guava leaves (psidium guajava L.) are known to have the potential as a source of natural antibiotics.
[2] Sembung vines contain bioactive compounds such as alkaloids, flavonoids, glycosides, tannins, and steroids/triterpenoids.
[3] Guava leaves contain flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids, which are known to have antibacterial activity.
[4] The use of natural antibiotics is becoming increasingly important due to the increasing problem of antibiotic resistance.
[5] Plants have been a rich source of bioactive compounds with antibacterial properties.
[6] Sembung vines and guava leaves are known to have the potential as a source of natural antibiotics.
[7] This study aims to identify the content of phytochemicals in dry powder and ethanol extracts of vines and guava leaves, as well as to test the effectiveness of the combination of ethanol extract of both types of leaves to *Escherichia coli *and *Bacillus cereus *.

Limitations

This study has some limitations. The study only tested the antibacterial activity of the combination of extracts on * Escherichia coli * and * Bacillus cereus *. Further research needs to be carried out to test the antibacterial activity of the combination of extracts on other bacteria.

Future Research Directions

Conclusion

In conclusion, the combination of ethanol extracts of Sembung C prophecy and guava leaves shows higher antibacterial activity compared to each single extract. These results open up opportunities for the development of natural antibacterials from plants that are safe and effective in dealing with diarrhea due to bacterial infections.
Q&A: Test Antibacterial Activity Combination of Ethanol Extracts Sembung C Prophecy (Mikania Micrantha Kunth) and Guava Leaves (Psidium Guajava L.) Against Escherichia coli and Bacillus cereus

Q: What is the purpose of this study?

A: The purpose of this study is to identify the content of phytochemicals in dry powder and ethanol extracts of Sembung C prophecy and guava leaves, as well as to test the effectiveness of the combination of ethanol extract of both types of leaves to *Escherichia coli *and *Bacillus cereus *.

Q: What are the potential benefits of using Sembung C prophecy and guava leaves as natural antibiotics?

A: Sembung C prophecy and guava leaves have been shown to have antibacterial properties, making them potential natural antibiotics. The combination of ethanol extracts of both plants may have a synergistic effect, increasing their antibacterial activity.

Q: What are the limitations of this study?

A: This study only tested the antibacterial activity of the combination of extracts on * Escherichia coli * and * Bacillus cereus *. Further research needs to be carried out to test the antibacterial activity of the combination of extracts on other bacteria.

Q: What are the potential applications of this study?

A: The results of this study can be the basis for the development of natural antibacterial products that are safe and effective in dealing with diarrhea and other bacterial infections.

Q: What are the next steps in this research?

A: Further research needs to be carried out to identify bioactive compounds that play a role in the synergistic effect, determine the mechanism of action, and test the toxicity of the combination of extracts in animal models.

Q: Why is it important to develop natural antibacterial products?

A: The increasing problem of antibiotic resistance makes it essential to develop new antibacterial products. Natural antibacterial products, such as those derived from Sembung C prophecy and guava leaves, may offer a safer and more effective alternative to traditional antibiotics.

Q: Can Sembung C prophecy and guava leaves be used as a treatment for other bacterial infections?

A: While this study only tested the antibacterial activity of the combination of extracts on * Escherichia coli * and * Bacillus cereus *, further research may be needed to determine their effectiveness against other bacterial infections.

Q: What are the potential risks associated with using Sembung C prophecy and guava leaves as natural antibiotics?

A: As with any new treatment, there may be potential risks associated with using Sembung C prophecy and guava leaves as natural antibiotics. Further research is needed to determine their safety and efficacy in humans.

Q: How can I access the full study?

A: The full study is available online through [insert link].