Potential Of Hexane Faction, Ethyl Acetate Butanol Pomegranate Skin Extract (punica Granatum) In The Process Of Alveolar Bone Remodeling For Prevention Of Relapse (as Self Retention) After The Movement Of Orthodontic Teeth
Potential of Pomegranate Skin Extract (Punica granatum) in the Process of Alveolar Bone Remodeling after Orthodontic Treatment
Introduction
Dental care using orthodontic devices is a common practice aimed at tidying the arrangement of teeth to achieve an ideal jaw's arch. However, this treatment process often leads to bone resorption around the teeth, which can cause relapse after the orthodontic treatment is complete. Pomegranate skin extract (punica granatum), rich in polyphenols, especially flavonoids and tannins, has been identified as a potential solution to prevent relapse after orthodontic treatment. This study highlights the potential of pomegranate skin extract in the process of alveolar bone remodeling and its application in preventing relapse after orthodontic treatment.
Bone and Relapse Remodeling Process
Bone remodeling is a natural process that involves the replacement of old bone cells with new ones. In the context of orthodontic treatment, this process is crucial, as the movement of teeth can change the structure of the surrounding alveolar bone. Several factors play a role in bone remodeling, including protein-protein interactions such as Collagen Type-1, BMP-2, and OsteoCalcin (OCN). These proteins function as mediators in the process of regulating bone formation and resorption.
Pomegranate skin extract is expected to affect protein expression, thereby increasing the process of bone remodeling and reducing the risk of dental relapse after orthodontic treatment. The potential of pomegranate skin extract in bone remodeling is attributed to its high content of polyphenols, which have been shown to have anti-inflammatory and antioxidant properties.
Research Methodology
This study employed a pure experimental design with a "Post Test Only Control Group" design involving 24 New Zealand White rabbits. The researchers divided the rabbits into four groups that received different treatment of pomegranate skin extract fractions formulated in the form of hydrogel. Before the experiment, the hydrogel was tested for biotolerance and immunogenesis activity against pathogenic cells.
Osteoblast cell viability activity test results in vitro showed that all extract fractions had a significant effect on cell activity (p <0.05) with moderate correlation (r = 0.6). Testing in Vivo with histopathological analysis showed that the butanol fraction of pomegranate skin extract could increase the expression of osteoblast and osteoclast cells at a certain concentration (125, 175, and 250 μg/ml).
Results and Discussion
The results showed that the butanol fraction of pomegranate skin extract not only accelerated alveolar bone remodeling but also affected the osteoblast cell histoscor profile significantly (p <0.05) with moderate correlation. Although the effect of the dose on osteoclast cells was also significant, the strength of the relationship was classified as very weak (r = 1.00).
The success of the butanol fraction in increasing the expression of Collagen Type-1, BMP-2, and OCN protein expressions shows that pomegranate skin extracts have the potential to be an additional therapy in orthodontic treatment, especially to prevent relapse. This therapy offers a new approach to dealing with classic problems in orthodontics that have been faced.
Conclusion
Pomegranate skin extract (punica granatum) shows a significant potential in improving the alveolar bone remodeling process and reducing the risk of relapse after active orthodontic treatment. The butanol fraction formulation in the form of hydrogel with a dose of 250 μg/ml is identified as an excellent choice to be applied in clinical practice. Further research is needed to explore the clinical applications of these findings and evaluate its long-term effects on dental and bone health.
Future Directions
Future studies should focus on exploring the clinical applications of pomegranate skin extract in orthodontic treatment. The effects of pomegranate skin extract on bone remodeling and relapse prevention should be evaluated in human subjects. Additionally, the long-term effects of pomegranate skin extract on dental and bone health should be investigated.
Limitations
This study had several limitations. The sample size was small, and the study was conducted in a controlled laboratory setting. The results may not be generalizable to human subjects. Additionally, the study only evaluated the effects of pomegranate skin extract on bone remodeling and relapse prevention, and did not investigate its effects on other aspects of orthodontic treatment.
Conclusion
In conclusion, pomegranate skin extract (punica granatum) shows a significant potential in improving the alveolar bone remodeling process and reducing the risk of relapse after active orthodontic treatment. The butanol fraction formulation in the form of hydrogel with a dose of 250 μg/ml is identified as an excellent choice to be applied in clinical practice. Further research is needed to explore the clinical applications of these findings and evaluate its long-term effects on dental and bone health.
Q&A: Potential of Pomegranate Skin Extract (Punica granatum) in the Process of Alveolar Bone Remodeling after Orthodontic Treatment
Frequently Asked Questions
Q: What is pomegranate skin extract, and how does it relate to orthodontic treatment?
A: Pomegranate skin extract is a natural compound derived from the skin of pomegranates. It is rich in polyphenols, especially flavonoids and tannins, which have been shown to have anti-inflammatory and antioxidant properties. In the context of orthodontic treatment, pomegranate skin extract has been identified as a potential solution to prevent relapse after orthodontic treatment.
Q: How does pomegranate skin extract affect bone remodeling?
A: Pomegranate skin extract is expected to affect protein expression, thereby increasing the process of bone remodeling and reducing the risk of dental relapse after orthodontic treatment. The potential of pomegranate skin extract in bone remodeling is attributed to its high content of polyphenols, which have been shown to have anti-inflammatory and antioxidant properties.
Q: What are the benefits of using pomegranate skin extract in orthodontic treatment?
A: The benefits of using pomegranate skin extract in orthodontic treatment include:
- Improved alveolar bone remodeling
- Reduced risk of dental relapse
- Anti-inflammatory and antioxidant properties
- Potential to be an additional therapy in orthodontic treatment
Q: How is pomegranate skin extract administered in orthodontic treatment?
A: Pomegranate skin extract is administered in the form of hydrogel, which is applied to the teeth and surrounding bone. The hydrogel is formulated with a specific concentration of pomegranate skin extract, which is tailored to the individual patient's needs.
Q: What are the potential side effects of using pomegranate skin extract in orthodontic treatment?
A: The potential side effects of using pomegranate skin extract in orthodontic treatment are minimal and include:
- Mild irritation or inflammation at the application site
- Temporary changes in taste or smell
- Rare cases of allergic reactions
Q: Can pomegranate skin extract be used in conjunction with other orthodontic treatments?
A: Yes, pomegranate skin extract can be used in conjunction with other orthodontic treatments, such as braces or clear aligners. However, the specific treatment plan and dosage of pomegranate skin extract will depend on the individual patient's needs and the severity of their orthodontic condition.
Q: How long does it take to see the effects of pomegranate skin extract in orthodontic treatment?
A: The effects of pomegranate skin extract in orthodontic treatment can be seen within a few weeks to a few months after treatment begins. However, the exact duration of treatment will depend on the individual patient's needs and the severity of their orthodontic condition.
Q: Is pomegranate skin extract safe for use in orthodontic treatment?
A: Yes, pomegranate skin extract is considered safe for use in orthodontic treatment. However, as with any medical treatment, there may be potential side effects or interactions with other medications. It is essential to consult with a qualified healthcare professional before using pomegranate skin extract in orthodontic treatment.
Conclusion
Pomegranate skin extract (punica granatum) has been identified as a potential solution to prevent relapse after orthodontic treatment. The benefits of using pomegranate skin extract in orthodontic treatment include improved alveolar bone remodeling, reduced risk of dental relapse, anti-inflammatory and antioxidant properties, and potential to be an additional therapy in orthodontic treatment. However, as with any medical treatment, there may be potential side effects or interactions with other medications. It is essential to consult with a qualified healthcare professional before using pomegranate skin extract in orthodontic treatment.