Metalloproteinase-9 Matrix Levels In New Zealand Rabbits With Chronic Spinal Cord Injury Model

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Introduction

Spinal cord injury (SCI) is a devastating medical condition that causes impaired function and psychological trauma due to fracture or spinal dislocation. Chronic SCI is a permanent or progressive disorder in impulse conduction along the neurons of the spinal cord. The pathophysiology of SCI involves complex mechanisms, including inflammation, oxidative stress, demyelination, and leukocyte infiltration. Matrix metalloproteinases (MMPs) play a crucial role in the degradation of extracellular matrix and protein, as well as extracellular matrix remodeling and wound healing. In this study, we investigated the levels of matrix metalloproteinase-9 (MMP-9) in New Zealand rabbits with chronic SCI models.

Background

MMP-9 is a member of the matrix metalloproteinase family, which is involved in the degradation of extracellular matrix and protein. MMP-9 has been shown to play a role in the pathophysiology of SCI, including inflammation, oxidative stress, demyelination, and leukocyte infiltration. The decrease in MMP-9 expression in post-SCI lumbar can help restore the learning ability of mice, suggesting that MMP-9 may have a complicated role in nerve improvement and cognitive function after SCI.

Methods

This study used an experimental laboratory analytic method in test animals and observations with a posttest only control group design. The test animals used were New Zealand rabbits, which were divided into three groups: group A (10, without compression handling), group B (10, with handling compression, MMP-9 was measured on the 14th day), and group C (10, with the handling of compression, MMP-9 was measured on the 21st day). The research approval was obtained from the Health Research Ethics Commission, University of North Sumatra with letter number 1034/Kepk/USU 2022. The statistical test used was the ANOVA test and then the Tukey test.

Results

The results showed that in the negative groups, MMP-9 levels were 2.44 to 0.88. There was an increase in the average MMP-9 in the positive group measured on the 14th day from 8.56 to 1.23 to 11.22 to 1.20 on the 21st day. Based on the analysis of the difference in average MMP-9 levels between groups, a significant difference was found between the examination time (p = <0.001). Therefore, the continued analysis of the Tukey test found a significant difference in the level of MMP-9 between the examination time in pairs (p = <0.001) in all group pairs.

Discussion

The increase in MMP-9 levels in New Zealand rabbits with chronic SCI models shows that MMP-9 plays an important role in the inflammatory process and tissue damage after SCI. The decrease in MMP-9 expression in post-SCI lumbar can help restore the learning ability of mice, suggesting that MMP-9 may have a complicated role in nerve improvement and cognitive function after SCI. This study provides important information about the role of MMP-9 in chronic SCI and can help develop new therapy for SCI in the future.

Conclusion

In conclusion, this study found an increase in the average level of MMP-9 in the experimental group of test animals that received the handling of spinal compression measured on the 21st day compared to the experimental group of animals without handling and handling compression measured on the 14th day.

Additional Analysis and Explanation

The increase in MMP-9 levels in New Zealand rabbits with chronic SCI models shows that MMP-9 plays an important role in the inflammatory process and tissue damage after SCI. The decrease in MMP-9 expression in post-SCI lumbar can help restore the learning ability of mice, suggesting that MMP-9 may have a complicated role in nerve improvement and cognitive function after SCI.

Limitations

This research has several limitations. First, this research uses the test animal model and may not necessarily be applied directly to humans. Second, the size of the sample of this research is relatively small, so the results of the study need to be confirmed with further research involving greater samples.

Future Research Directions

Further research is needed to understand the role of MMP-9 in the pathophysiology of SCI and the potential use of MMP-9 as a therapy target for SCI.

Useful Resources

This study provides important information about the role of MMP-9 in chronic SCI and can help develop new therapy for SCI in the future.

Frequently Asked Questions

We have received many questions from readers about our recent study on the increased levels of matrix metalloproteinase-9 (MMP-9) in New Zealand rabbits with chronic spinal cord injury models. Here are some of the most frequently asked questions and our answers:

Q: What is the purpose of this study?

A: The purpose of this study is to investigate the levels of matrix metalloproteinase-9 (MMP-9) in New Zealand rabbits with chronic spinal cord injury models. We wanted to understand the role of MMP-9 in the inflammatory process and tissue damage after spinal cord injury.

Q: What is MMP-9 and how does it relate to spinal cord injury?

A: MMP-9 is a member of the matrix metalloproteinase family, which is involved in the degradation of extracellular matrix and protein. In the context of spinal cord injury, MMP-9 has been shown to play a role in the inflammatory process and tissue damage.

Q: What were the results of the study?

A: The results of the study showed that there was an increase in the average level of MMP-9 in the experimental group of test animals that received the handling of spinal compression measured on the 21st day compared to the experimental group of animals without handling and handling compression measured on the 14th day.

Q: What are the implications of this study?

A: The implications of this study are that MMP-9 may play an important role in the inflammatory process and tissue damage after spinal cord injury. This suggests that MMP-9 may be a potential target for therapy in the treatment of spinal cord injury.

Q: What are the limitations of this study?

A: The limitations of this study include the use of a test animal model, which may not necessarily be applied directly to humans. Additionally, the size of the sample of this research is relatively small, so the results of the study need to be confirmed with further research involving greater samples.

Q: What are the future research directions?

A: Further research is needed to understand the role of MMP-9 in the pathophysiology of spinal cord injury and the potential use of MMP-9 as a therapy target for spinal cord injury.

Q: What resources are available for further reading?

A: There are several resources available for further reading on the topic of MMP-9 and spinal cord injury, including:

We hope that this Q&A article has provided helpful information and answers to your questions about our recent study on the increased levels of matrix metalloproteinase-9 (MMP-9) in New Zealand rabbits with chronic spinal cord injury models.