Isolation And Identification Of Phenolic Compounds From The Bark Of The Stem Of The Kalpataru Plant (rah Crepitance Linn.)
Introduction
Kalpataru (Hura Crepitans Linn.), a tree with a beautiful name and efficacy that is no less interesting, stores great potential in the world of treatment. This tree bark is known to contain phenolic compounds, which are known to have diverse biological activity, such as antioxidants, anti-inflammatory, and antibacterial. This study aims to isolate and identify phenolic compounds contained in the bark of Kalpataru, opening a veil about the potential benefits for human health.
Phenolic compounds are a class of compounds that are widely distributed in plants and have been shown to have various biological activities. They are known to have antioxidant, anti-inflammatory, and antibacterial properties, making them potential candidates for the treatment of various diseases. The Kalpataru tree, with its unique properties, is a potential source of these compounds.
Materials and Methods
The isolation process begins with extraction using methanol as a solvent. Methanol extract is then extracted with ethyl acetate repeatedly so that it does not show a positive reaction with a 5%FeCl3. The ethyl acetate extract obtained is then concentrated and dissolved again in methanol, then partitioned with N-hexane until the N-HEKEXANA layer is clear. Methanol extracts that are left then separated by column chromatography using the Silica Gel Phase and Chloroform Motion phase: Ethyl acetate (90:10; 80:20; 70:30; 60:40 50:50; 40:60) V/V.
The fraction obtained from the process (fraction 131-190) is then purified using a preparative thin layer chromatography. The result is 12 mg of yellow amorphous solids with RF value of 0.44 using chloroform: methanol (90:10) V/V as eluent. Identification of successful isolated compounds is carried out using UV-Vis, FTIR, and 1H-NMR spectrophotometers.
Results and Discussion
The UV-Vis spectrophotometer shows that the compound has a maximum wavelength (λ max) 217 ​​Nm of band II and 275 nm in the band I, which is a typical characteristic of phenolic compounds. FTIR analysis shows the presence of groups -OH, CH, C = O, C = C, C-H, and C-O on the spectrum, reinforcing the allegation that the compound is a phenolic compound.
1H-NMR spectroscopy reveals more detailed information about the structure of compounds. The presence of Proton H-2 and H-6 on the aromatic ring, Proton H-2 'from CH3 and Proton H-4 from CH3 bound to the benzene ring, and Proton H-1' from OCH2 and Proton H from CH2 bound to the ring Aromatic indicates that the compound is likely to be a derivative of phenilastic acid.
Conclusion
This study provides a promising initial result of the potential of phenolic compounds in kalpataru bark. Although further research is still needed to identify compounds with certainty, the results of the initial analysis indicate that the compound is likely to be a derivative of phenilastic acid. This finding opens up opportunities for developing the potential of Kalpataru as a source of bioactive compounds with significant health benefits.
Future Directions
Further research is needed to identify the exact structure of the compound and to investigate its biological activities. Additionally, the potential of Kalpataru as a source of bioactive compounds should be explored further. This study provides a promising initial result, and further research is needed to confirm the potential of Kalpataru as a source of bioactive compounds.
References
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- [2] [Insert reference 2]
- [3] [Insert reference 3]
Note: The references should be cited in the text and listed at the end of the article.
Limitations
This study has some limitations. The isolation and identification of phenolic compounds from the bark of Kalpataru is a complex process, and the results may not be representative of the entire plant. Additionally, the biological activities of the compound are not fully understood, and further research is needed to confirm its potential as a bioactive compound.
Conclusion
In conclusion, this study provides a promising initial result of the potential of phenolic compounds in kalpataru bark. Although further research is still needed to identify compounds with certainty, the results of the initial analysis indicate that the compound is likely to be a derivative of phenilastic acid. This finding opens up opportunities for developing the potential of Kalpataru as a source of bioactive compounds with significant health benefits.
Q: What is Kalpataru and why is it of interest?
A: Kalpataru (Hura Crepitans Linn.) is a tree with a beautiful name and efficacy that is no less interesting. It is known to contain phenolic compounds, which are known to have diverse biological activity, such as antioxidants, anti-inflammatory, and antibacterial.
Q: What are phenolic compounds and what are their benefits?
A: Phenolic compounds are a class of compounds that are widely distributed in plants and have been shown to have various biological activities. They are known to have antioxidant, anti-inflammatory, and antibacterial properties, making them potential candidates for the treatment of various diseases.
Q: What is the purpose of this study?
A: The purpose of this study is to isolate and identify phenolic compounds contained in the bark of Kalpataru, opening a veil about the potential benefits for human health.
Q: What methods were used to isolate and identify the phenolic compounds?
A: The isolation process begins with extraction using methanol as a solvent. Methanol extract is then extracted with ethyl acetate repeatedly so that it does not show a positive reaction with a 5%FeCl3. The ethyl acetate extract obtained is then concentrated and dissolved again in methanol, then partitioned with N-hexane until the N-HEKEXANA layer is clear. Methanol extracts that are left then separated by column chromatography using the Silica Gel Phase and Chloroform Motion phase: Ethyl acetate (90:10; 80:20; 70:30; 60:40 50:50; 40:60) V/V.
Q: What were the results of the UV-Vis spectrophotometer analysis?
A: The UV-Vis spectrophotometer shows that the compound has a maximum wavelength (λ max) 217 ​​Nm of band II and 275 nm in the band I, which is a typical characteristic of phenolic compounds.
Q: What were the results of the FTIR analysis?
A: FTIR analysis shows the presence of groups -OH, CH, C = O, C = C, C-H, and C-O on the spectrum, reinforcing the allegation that the compound is a phenolic compound.
Q: What were the results of the 1H-NMR spectroscopy?
A: 1H-NMR spectroscopy reveals more detailed information about the structure of compounds. The presence of Proton H-2 and H-6 on the aromatic ring, Proton H-2 'from CH3 and Proton H-4 from CH3 bound to the benzene ring, and Proton H-1' from OCH2 and Proton H from CH2 bound to the ring Aromatic indicates that the compound is likely to be a derivative of phenilastic acid.
Q: What are the potential applications of the isolated compound?
A: The isolated compound has potential applications in the development of bioactive compounds with significant health benefits.
Q: What are the limitations of this study?
A: This study has some limitations. The isolation and identification of phenolic compounds from the bark of Kalpataru is a complex process, and the results may not be representative of the entire plant. Additionally, the biological activities of the compound are not fully understood, and further research is needed to confirm its potential as a bioactive compound.
Q: What are the future directions of this research?
A: Further research is needed to identify the exact structure of the compound and to investigate its biological activities. Additionally, the potential of Kalpataru as a source of bioactive compounds should be explored further.
Q: What are the implications of this study for the development of new medicines?
A: This study provides a promising initial result of the potential of phenolic compounds in kalpataru bark. Although further research is still needed to identify compounds with certainty, the results of the initial analysis indicate that the compound is likely to be a derivative of phenilastic acid. This finding opens up opportunities for developing the potential of Kalpataru as a source of bioactive compounds with significant health benefits.