Induction Of Black Stilt Buds (curcuma Aeruginosa Roxb.) With The Administration Of BAP And NAA Through Vitro Culture

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Induction of Black Stilt Buds (Curcuma Aeruginosa Roxb.) with the Administration of BAP and NAA through In Vitro Culture

Introduction

In the field of plant biology, in vitro culture has emerged as a powerful tool for the propagation and improvement of plant species. This technique involves the growth of plant cells, tissues, or organs in a controlled environment outside of the plant's natural habitat. One of the key challenges in in vitro culture is the induction of shoots and roots, which is crucial for the successful propagation of plants. In this study, we aimed to investigate the effect of BAP (Benzylaminopurine) and NAA (naphthalenecetic acid) on the induction of black stilt buds (Curcuma aeruginosa Roxb.) through in vitro culture.

Background

Black stilt (Curcuma aeruginosa Roxb.) is a rare and endangered plant species that is highly valued for its medicinal properties. However, its limited availability and high demand have made it a challenging species to propagate. In vitro culture offers a promising solution for the mass production of black stilt plants, but the development of effective protocols for shoot and root induction is essential for the success of this technique.

Materials and Methods

The study was conducted in the Alifa Agricultural Research Center (Alifa-Arc) Laboratory in Medan from June to October 2022. The experimental design used was a Complete Random Design (RAL) with two treatment factors, namely BAP Concentration (0 mg/L, 2 mg/L, 4 mg/L, 6 mg/L) and NAA concentration (0 mg/L, 0.5 mg/L, 1 mg/L). The results showed that the combination of BAP and NAA can increase the number of black stilt shoots more than one. The combination that produces the longest shoot length is at a concentration of 6 mg/L BAP and 0.5 mg/L NAA, while the shortest shoot length occurs at a concentration of 0 mg/L BAP and 1 mg/L NAA.

Results

The results of this study showed that the combination of BAP and NAA can significantly affect the growth of black stilt shoots. The combination of 6 mg/L BAP and 0.5 mg/L NAA produced the longest shoot length, while the combination of 0 mg/L BAP and 1 mg/L NAA produced the shortest shoot length. However, the combination of BAP and NAA did not increase root induction in black stilt plants. New roots can be induced if there are BAP and NAA in MS media.

Discussion

The use of plant hormones such as BAP and NAA is crucial in in vitro culture for stimulating the growth of shoots and roots. BAP is a cytokinin that plays a role in cell division and shoot development, while NAA is auxin that functions in the formation of roots. The combination of these two hormones allows researchers to manipulate plant growth in accordance with the research objectives.

The combination of 6 mg/L BAP and 0.5 mg/L NAA is proven effective in producing longer shoots, which shows that an increase in BAP concentration contributes significantly in accelerating the growth of shoots. This is important for practical applications in plant breeding, especially for rare plants or those that have high economic value such as black stilt. In the agricultural industry, the development of in vitro culture techniques also has the potential to increase productivity and quality of agricultural products.

However, it is essential to note that although this combination is good for shoot growth, root induction remains a challenge. Further research is needed to explore other hormonal concentrations and media conditions that may be more effective in supporting root growth. Thus, this research not only provides insight into the process of induction of the shoot but also opens opportunities for further research in the development of in vitro culture methods for other plants.

Conclusion

In conclusion, this study shows that the combination of BAP and NAA can affect the growth of shoots in black stilt plants, but to achieve optimal root growth, additional research is needed regarding media formulations and other hormone concentrations. Innovation and ongoing research in the field of plant tissue culture is very important to support food security and agricultural sustainability in the future.

Recommendations

Based on the results of this study, the following recommendations are made:

  1. Further research is needed to explore other hormonal concentrations and media conditions that may be more effective in supporting root growth.
  2. The development of in vitro culture techniques for black stilt plants should be continued to improve the efficiency and productivity of this technique.
  3. The use of BAP and NAA in in vitro culture should be further investigated to determine their optimal concentrations and combinations for different plant species.

Future Directions

The results of this study have opened up new avenues for research in the field of plant tissue culture. Future studies should focus on the following areas:

  1. The development of in vitro culture protocols for other plant species that are difficult to propagate.
  2. The investigation of the effects of different hormonal concentrations and combinations on plant growth and development.
  3. The development of new media formulations and culture conditions that can support optimal root growth and plant development.

By continuing to explore the possibilities of in vitro culture, we can improve our understanding of plant biology and develop new techniques for the propagation and improvement of plant species.
Frequently Asked Questions (FAQs) about Induction of Black Stilt Buds (Curcuma Aeruginosa Roxb.) with the Administration of BAP and NAA through In Vitro Culture

Q: What is in vitro culture?

A: In vitro culture is a technique used to grow plant cells, tissues, or organs in a controlled environment outside of the plant's natural habitat. This technique is used to propagate and improve plant species.

Q: What is the purpose of this study?

A: The purpose of this study is to investigate the effect of BAP (Benzylaminopurine) and NAA (naphthalenecetic acid) on the induction of black stilt buds (Curcuma aeruginosa Roxb.) through in vitro culture.

Q: What are BAP and NAA?

A: BAP (Benzylaminopurine) is a cytokinin that plays a role in cell division and shoot development, while NAA (naphthalenecetic acid) is an auxin that functions in the formation of roots.

Q: What were the results of this study?

A: The results of this study showed that the combination of BAP and NAA can increase the number of black stilt shoots more than one. The combination that produces the longest shoot length is at a concentration of 6 mg/L BAP and 0.5 mg/L NAA, while the shortest shoot length occurs at a concentration of 0 mg/L BAP and 1 mg/L NAA.

Q: Why is root induction a challenge in this study?

A: Root induction is a challenge in this study because the combination of BAP and NAA did not increase root induction in black stilt plants. New roots can be induced if there are BAP and NAA in MS media.

Q: What are the implications of this study?

A: The implications of this study are that the combination of BAP and NAA can affect the growth of shoots in black stilt plants, but to achieve optimal root growth, additional research is needed regarding media formulations and other hormone concentrations.

Q: What are the future directions of this research?

A: The future directions of this research are to further investigate the effects of different hormonal concentrations and combinations on plant growth and development, and to develop new media formulations and culture conditions that can support optimal root growth and plant development.

Q: Why is in vitro culture important?

A: In vitro culture is important because it offers a promising solution for the mass production of plant species that are difficult to propagate. This technique can also be used to improve plant growth and development, and to develop new plant varieties with desirable traits.

Q: What are the benefits of in vitro culture?

A: The benefits of in vitro culture include:

  • Improved plant growth and development
  • Increased productivity and quality of agricultural products
  • Development of new plant varieties with desirable traits
  • Mass production of plant species that are difficult to propagate

Q: What are the limitations of in vitro culture?

A: The limitations of in vitro culture include:

  • The need for specialized equipment and facilities
  • The requirement for skilled personnel to perform the technique
  • The potential for contamination and other technical issues
  • The need for further research to improve the efficiency and productivity of the technique.