In Vitro Test Antimicrobial Activity Of Batak Tubers Extract (Allium Chinense G. Don.) Against Methicillin-resistant Staphylococcus Aureus

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In Vitro Test Antimicrobial Activity of Batak Tubers Extract (Allium Chinense G. Don.) Against Methicillin-Resistant Staphylococcus Aureus

Introduction

Infections caused by Staphylococcus aureus can lead to pioderma conditions, which include folliculitis, furunculosis, carbuncle, and impetigo. In Indonesia, pioderma is still a common health problem. In recent years, there have been significant changes in the sensitivity of Staphylococcus aureus to antibiotics, which are characterized by the emergence of meticillin (methicillin-resistant staphylococcus aureus or MRSA) strains. This requires alternative treatment, especially in the form of herbal medicines. The use of herbal medicines has been increasing in recent years due to their potential to provide effective treatment without the side effects associated with conventional antibiotics.

In Indonesia, there are various medicinal plants, but the use is still minimal. One of the genus of plants that has been known to have antimicrobial activity is allium. One type, Bawang Batak (Allium Chinense G. Don.), is very popular among the people, especially in North Sumatra. Although research on the antimicrobial activity of the Batak onion has been carried out before, there is no research that focuses on its influence on MRSA. Therefore, this study aims to assess the antimicrobial activity of the Batak onion extract in various concentrations of MRSA bacteria. The study aims to provide insight into the potential of Batak onion extract as an alternative therapy for MRSA infections.

Methodology

This research is an experimental study that uses the agar diffusion method. The onion extract is divided into six groups with different concentrations: 6.25%, 12.5%, 25%, 50%, 100%, and one negative control group using DMSO. Each group was tested four times, so that the total samples analyzed were 24. The agar diffusion method is a widely used technique for testing the antimicrobial activity of plant extracts.

Research Result

The results showed that inhibition of bacterial growth occurred at 50% extract concentrations with a diameter of an inhibitory zone of 8.695 mm and at a concentration of 100% with a diameter of the inhibition zone of 10.545 mm. Conversely, extracts at a concentration of 25%, 12.5%, and 6.25% do not show inhibitory activity to the growth of MRSA bacteria. The results indicate that the Batak onion extract has antimicrobial activity against MRSA bacteria, especially at higher concentrations.

Conclusion

Based on the results obtained, it can be concluded that the Batak onion tubers extract has the potential as an MRSA growth inhibiting agent, especially at higher concentrations (50% and 100%). This discovery opens opportunities for the development of herbal-based alternative medicine in dealing with infections caused by resistant bacteria, such as MRSA. This is very important to note given the uncertainty and challenges faced by the medical world in dealing with conventional antibiotic bacteria.

Benefits of Research

This study not only provides insight into the potential of Batak onion extract as an alternative therapy for MRSA infections, but also emphasizes the importance of further research to maximize the use of local medicinal plants in Indonesia. The community needs to be given sufficient information about the benefits of herbal plants around them, so they can use these natural resources more effectively for health.

Thus, further exploration of the antimicrobial properties of traditional medicinal plants such as Batak onions is expected to make a significant contribution to the development of future health therapy. The study highlights the potential of Batak onion extract as a natural remedy for MRSA infections and emphasizes the need for further research in this area.

Future Directions

The study suggests that further research is needed to explore the antimicrobial properties of Batak onion extract and to develop it as a potential alternative therapy for MRSA infections. The study also highlights the need for further research on the safety and efficacy of Batak onion extract in humans.

In conclusion, the study provides insight into the potential of Batak onion extract as an alternative therapy for MRSA infections and emphasizes the importance of further research in this area. The study highlights the potential of Batak onion extract as a natural remedy for MRSA infections and emphasizes the need for further research in this area.

Recommendations

Based on the results of this study, the following recommendations are made:

  1. Further research is needed to explore the antimicrobial properties of Batak onion extract and to develop it as a potential alternative therapy for MRSA infections.
  2. The safety and efficacy of Batak onion extract in humans need to be further investigated.
  3. The community needs to be given sufficient information about the benefits of herbal plants around them, so they can use these natural resources more effectively for health.

Conclusion

In conclusion, the study provides insight into the potential of Batak onion extract as an alternative therapy for MRSA infections and emphasizes the importance of further research in this area. The study highlights the potential of Batak onion extract as a natural remedy for MRSA infections and emphasizes the need for further research in this area.

References

  1. World Health Organization. (2019). Antimicrobial Resistance: Global Report on Surveillance.
  2. Centers for Disease Control and Prevention. (2020). Methicillin-Resistant Staphylococcus aureus (MRSA).
  3. National Institute of Health. (2020). Antimicrobial Resistance.

Appendix

The appendix includes the following:

  1. Table 1: Inhibition zone diameter of Batak onion extract against MRSA bacteria.
  2. Table 2: Concentration of Batak onion extract used in the study.
  3. Figure 1: Inhibition zone diameter of Batak onion extract against MRSA bacteria.
  4. Figure 2: Concentration of Batak onion extract used in the study.
    Frequently Asked Questions (FAQs) About In Vitro Test Antimicrobial Activity of Batak Tubers Extract (Allium Chinense G. Don.) Against Methicillin-Resistant Staphylococcus Aureus

Q: What is the purpose of this study?

A: The purpose of this study is to assess the antimicrobial activity of Batak onion extract in various concentrations of MRSA bacteria. The study aims to provide insight into the potential of Batak onion extract as an alternative therapy for MRSA infections.

Q: What is MRSA?

A: MRSA (Methicillin-Resistant Staphylococcus aureus) is a type of bacteria that is resistant to many antibiotics. MRSA infections can be difficult to treat and can cause a range of symptoms, from mild to severe.

Q: What is Batak onion extract?

A: Batak onion extract is a natural remedy that is derived from the Batak onion plant (Allium Chinense G. Don.). The extract has been traditionally used in Indonesia to treat various health conditions, including infections.

Q: What are the benefits of using Batak onion extract as an alternative therapy for MRSA infections?

A: The benefits of using Batak onion extract as an alternative therapy for MRSA infections include:

  • Natural and non-toxic: Batak onion extract is a natural remedy that is non-toxic and free from side effects.
  • Effective: The study found that Batak onion extract has antimicrobial activity against MRSA bacteria, especially at higher concentrations.
  • Cost-effective: Batak onion extract is a cost-effective alternative to conventional antibiotics.

Q: What are the limitations of this study?

A: The limitations of this study include:

  • Small sample size: The study had a small sample size, which may limit the generalizability of the results.
  • In vitro study: The study was conducted in vitro, which may not reflect the in vivo situation.
  • Need for further research: Further research is needed to confirm the results of this study and to explore the safety and efficacy of Batak onion extract in humans.

Q: What are the future directions for this research?

A: The future directions for this research include:

  • In vivo studies: Conducting in vivo studies to confirm the results of this study and to explore the safety and efficacy of Batak onion extract in humans.
  • Clinical trials: Conducting clinical trials to evaluate the efficacy and safety of Batak onion extract as an alternative therapy for MRSA infections.
  • Development of Batak onion extract as a natural remedy: Developing Batak onion extract as a natural remedy for MRSA infections and other health conditions.

Q: How can I access the full text of this study?

A: The full text of this study is available online through various academic databases, including Google Scholar and ResearchGate. You can also contact the author directly to request a copy of the study.

Q: What are the implications of this study for public health?

A: The implications of this study for public health include:

  • Alternative therapy for MRSA infections: Batak onion extract may provide an alternative therapy for MRSA infections, which can be difficult to treat with conventional antibiotics.
  • Natural remedy for infections: Batak onion extract may be used as a natural remedy for various infections, including MRSA infections.
  • Promoting the use of natural remedies: This study highlights the potential of natural remedies, such as Batak onion extract, as an alternative to conventional antibiotics.