Identification Of Genetic Similarities In Several Genotypes Of Saccharum Spp. North Sumatra With Variety Of Sugarcane Sugar Cane Varieties (PS 864 And PSJT 941) Using Random Amplified Polymorphism DNA Markers

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Identification of Genetic Similarities in Several Genotypes of Saccharum SPP. North Sumatra with Variety of Sugarcane Sugar Cane Varieties (PS 864 and PSJT 941) Using Random Amplified Polymorphism DNA Markers

Introduction

The identification of genetic similarities in several genotypes of Saccharum SPP. North Sumatra with variety of sugarcane sugar cane varieties (PS 864 and PSJT 941) is a crucial step in developing superior sugar cane varieties that are resistant to drought. The increasing extreme climate change has a significant impact on the availability of water for plants, making it essential to develop drought-tolerant sugar cane varieties. This study aims to identify the genetic similarity of several Saccharum SPP. sugarcane genotypes from North Sumatra with a variety of sugarcane cane drought PS 864 and PSJT 941 using Random Amplified Polymorphism DNA (RAPD) markers.

Materials and Methods

This study used RAPD markers to analyze DNA from 30 North Sumatra sugar cane accessions. A total of 14 primary discrining primers were used, of which 10 primers succeeded in collapsing DNA and producing reproduced DNA fragments. The analysis of DNA fragments was performed using gel electrophoresis, and the results were analyzed using the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method.

Results

The analysis of DNA fragments using RAPD markers resulted in a total of 70 polymorphic bands, with the number of amplification products per primer varying from 5 (OPN 03) to 9 (OPA 04, OPC 18, OPD 03). The DNA fragments ranged from 196 to 1973 base pairs (BP). The average percentage of polymorphics and polymorphic information content (PIC) of the 10 primers used was 98.75% and 0.454, respectively. This indicates that RAPD markers are effective in distinguishing genetic diversity in sugarcane genotypes studied.

Discussion

The analysis of genetic and dendogram distances resulted in the division of 30 North Sumatra sugar cane accessions into three groups (clusters). The genetic distance between accessions varied, ranging from 0.067 (Silver/Sunggal Gambas Gambas) to 0.783 (Helvetia/Berastagi Sibolangit field). Interestingly, the variety of sugarcane tolerant of drought PS 864 and PSJT 941 was in the same cluster. This suggests that some North Sumatra sugar cane genotypes have a genetic similarity that is quite close to the tolerant variety of drought PS 864 and PSJT 941.

Conclusion

The results of this study indicate that some North Sumatra sugar cane genotypes have a genetic similarity that is quite close to the tolerant variety of drought PS 864 and PSJT 941. PS 882 sugar cane genotype has the closest genetic similarity to PS 864 (the similarity coefficient of 0.828), while Kidang Kencana has the most genetic similarity close to PSJT 941 (similarity coefficient of 0.787). This finding opens great opportunities to utilize the local sugar cane genotype in North Sumatra in the sugar cane breeding program. Sugar cane genotype which has a genetic similarity with a large potential drought-tolerant variety to be developed into a superior variety that is resistant to drought.

Implications

This study highlights the importance of exploration and utilization of local sugar cane genetic diversity. Indonesian local sugar cane holds great genetic potential, including resistance to drought. The development of superior sugar cane varieties based on local genetic resources will support food security and improve the welfare of sugar cane farmers in Indonesia. The increasing extreme climate change has a significant impact on the availability of water for plants, making it essential to develop drought-tolerant sugar cane varieties.

Future Directions

This study provides a foundation for further research on the development of superior sugar cane varieties that are resistant to drought. The identification of genetic similarities in several genotypes of Saccharum SPP. North Sumatra with variety of sugarcane sugar cane varieties (PS 864 and PSJT 941) using RAPD markers is a crucial step in developing drought-tolerant sugar cane varieties. Future studies should focus on the development of superior sugar cane varieties based on local genetic resources and the evaluation of their performance under drought conditions.

Recommendations

Based on the results of this study, the following recommendations are made:

  1. Utilization of local sugar cane genetic diversity: The local sugar cane genotype in North Sumatra has great genetic potential, including resistance to drought. The utilization of this genetic diversity is essential for the development of superior sugar cane varieties.
  2. Development of drought-tolerant sugar cane varieties: The development of drought-tolerant sugar cane varieties is essential for supporting food security and improving the welfare of sugar cane farmers in Indonesia.
  3. Evaluation of sugar cane varieties under drought conditions: The evaluation of sugar cane varieties under drought conditions is essential for identifying the most suitable varieties for cultivation in drought-prone areas.

Conclusion

In conclusion, this study provides a foundation for further research on the development of superior sugar cane varieties that are resistant to drought. The identification of genetic similarities in several genotypes of Saccharum SPP. North Sumatra with variety of sugarcane sugar cane varieties (PS 864 and PSJT 941) using RAPD markers is a crucial step in developing drought-tolerant sugar cane varieties. The utilization of local sugar cane genetic diversity and the development of drought-tolerant sugar cane varieties are essential for supporting food security and improving the welfare of sugar cane farmers in Indonesia.
Q&A: Identification of Genetic Similarities in Several Genotypes of Saccharum SPP. North Sumatra with Variety of Sugarcane Sugar Cane Varieties (PS 864 and PSJT 941) Using Random Amplified Polymorphism DNA Markers

Q: What is the main objective of this study?

A: The main objective of this study is to identify the genetic similarity of several Saccharum SPP. sugarcane genotypes from North Sumatra with a variety of sugarcane cane drought PS 864 and PSJT 941 using Random Amplified Polymorphism DNA (RAPD) markers.

Q: What is RAPD and how does it work?

A: RAPD is a molecular marker technique that uses short primers to amplify specific regions of the DNA. The amplified DNA fragments are then separated by gel electrophoresis and analyzed to identify polymorphisms. In this study, RAPD markers were used to analyze DNA from 30 North Sumatra sugar cane accessions.

Q: What are the benefits of using RAPD markers in this study?

A: The use of RAPD markers in this study allowed for the identification of genetic diversity in sugarcane genotypes and the detection of polymorphisms. This information is essential for developing superior sugar cane varieties that are resistant to drought.

Q: What are the implications of this study for the development of drought-tolerant sugar cane varieties?

A: The results of this study indicate that some North Sumatra sugar cane genotypes have a genetic similarity that is quite close to the tolerant variety of drought PS 864 and PSJT 941. This suggests that these genotypes can be used as a starting material for the development of drought-tolerant sugar cane varieties.

Q: What are the potential applications of this study?

A: The results of this study can be used to develop drought-tolerant sugar cane varieties that are resistant to drought. This can help to improve food security and the welfare of sugar cane farmers in Indonesia.

Q: What are the limitations of this study?

A: One limitation of this study is that it only analyzed DNA from 30 North Sumatra sugar cane accessions. Future studies should aim to analyze a larger number of accessions to confirm the results of this study.

Q: What are the future directions for this research?

A: Future studies should focus on the development of superior sugar cane varieties based on local genetic resources and the evaluation of their performance under drought conditions.

Q: What are the recommendations for future research?

A: Based on the results of this study, the following recommendations are made:

  1. Utilization of local sugar cane genetic diversity: The local sugar cane genotype in North Sumatra has great genetic potential, including resistance to drought. The utilization of this genetic diversity is essential for the development of superior sugar cane varieties.
  2. Development of drought-tolerant sugar cane varieties: The development of drought-tolerant sugar cane varieties is essential for supporting food security and improving the welfare of sugar cane farmers in Indonesia.
  3. Evaluation of sugar cane varieties under drought conditions: The evaluation of sugar cane varieties under drought conditions is essential for identifying the most suitable varieties for cultivation in drought-prone areas.

Q: What are the conclusions of this study?

A: In conclusion, this study provides a foundation for further research on the development of superior sugar cane varieties that are resistant to drought. The identification of genetic similarities in several genotypes of Saccharum SPP. North Sumatra with variety of sugarcane sugar cane varieties (PS 864 and PSJT 941) using RAPD markers is a crucial step in developing drought-tolerant sugar cane varieties. The utilization of local sugar cane genetic diversity and the development of drought-tolerant sugar cane varieties are essential for supporting food security and improving the welfare of sugar cane farmers in Indonesia.