Formulation And Test Of Antibacterial Activity Nanocrim Preparation Ethanol Extract Of Honey Guava (Syzygium Samaranene (Blume) Merr. & L.M Perry) In The Bacterium Propionibacterium Acnes And Staphylococcus Epidermidis
Introduction
Acne is a common skin condition that affects millions of people worldwide, often caused by bacterial infections. One of the natural solutions that promises to overcome this problem is to use ethanol extract from honey guava fruit (Syzygium samaranene (Blume) Merr. & L.M Perry). This fruit is rich in bioactive compounds such as flavonoids, tannins, and saponins which have antibacterial properties. The use of ethanol extract from honey guava has been gaining attention in recent years due to its potential in treating various skin conditions, including acne. This study aims to formulate the ethanol extract of honey guava fruit into the form of nanocrim and evaluate its antibacterial activity, as well as comparing it with the preparation of creams against propionibacterium acnes and staphylococcus epidermidis bacteria.
Research Purposes
The primary objective of this study is to develop a nanocrim formulation of ethanol extract from honey guava fruit and evaluate its antibacterial activity against propionibacterium acnes and staphylococcus epidermidis bacteria. The study also aims to compare the antibacterial activity of the nanocrim formulation with that of cream preparations. This research is significant as it provides new insights into the development of natural ingredient-based dermatological products that can be utilized in skin care.
Methodology
The ethanol extract from honey guava fruit was made using the maceration method. The nanocrim formulation was developed using tween 80 (36%) as a surfactant and peg 400 (6%) as a cosurfactant, with varying extract concentrations, namely 2.5% (F1), 5% (F2), and 7.5% (F3). Evaluation of the physical characteristics of the preparation includes particle size tests, homogeneity, emulsion type, surface tension, spread power, and physical stability for 12 weeks at various temperatures. In addition, an antibacterial activity testing is also carried out to determine the ability of extracts and nanocrim in inhibiting bacterial growth.
Research Result
The results show that the three formulas of nanocrim (F1, F2, F3) have good physical characteristics, with high homogeneity and Oil-in-Water emulsion type (O/W). The measured surface tension ranges from 33.3 to 51.2 dyne/cm, as well as spreading power between 5.1 to 6.7 cm. Nanocrim also shows good stability in the centrifugation test and is stable for storage for 12 weeks at different temperatures (4 ± 2 ℃, 25 ± 2 ℃, and 40 ± 2 ℃).
Nanocrim F3 (with an extract concentration of 7.5%) shows the most powerful antibacterial activity, with the diameter of the inhibition zone to propionibacterium acnes and staphylococcus epidermidis each of 16.47 ± 0.378 mm and 16.33 ± 0.378 mm. Meanwhile, cream preparations only show the diameter of the inhibitory zone of 9.27 ± 0.960 mm and 11.13 ± 0.321 mm.
Conclusion
Ethanol extract of honey guava fruit can be successfully formulated into a nanocrim preparation that meets the evaluation requirements. Nanocrim formula with a concentration of 7.5% shows a significant antibacterial ability to be higher than other formulas and also compared to cream preparations. This shows the great potential for using nanocrim-based honey guava fruit as an alternative in the treatment of acne that is more effective. This study provides new insights in the development of natural ingredient-based dermatological products that can be utilized in skin care.
Discussion
The results of this study demonstrate the potential of ethanol extract from honey guava fruit as a natural ingredient in the treatment of acne. The nanocrim formulation of ethanol extract from honey guava fruit shows good physical characteristics and antibacterial activity against propionibacterium acnes and staphylococcus epidermidis bacteria. The results of this study are significant as they provide new insights into the development of natural ingredient-based dermatological products that can be utilized in skin care.
Limitation
This study has some limitations. The study only evaluated the antibacterial activity of the nanocrim formulation against two types of bacteria, propionibacterium acnes and staphylococcus epidermidis. Further studies are needed to evaluate the antibacterial activity of the nanocrim formulation against other types of bacteria. Additionally, the study only evaluated the physical characteristics of the nanocrim formulation, and further studies are needed to evaluate the stability and shelf life of the nanocrim formulation.
Future Direction
The results of this study demonstrate the potential of ethanol extract from honey guava fruit as a natural ingredient in the treatment of acne. Further studies are needed to evaluate the antibacterial activity of the nanocrim formulation against other types of bacteria and to evaluate the stability and shelf life of the nanocrim formulation. Additionally, further studies are needed to evaluate the efficacy of the nanocrim formulation in treating acne in humans.
Conclusion
In conclusion, this study demonstrates the potential of ethanol extract from honey guava fruit as a natural ingredient in the treatment of acne. The nanocrim formulation of ethanol extract from honey guava fruit shows good physical characteristics and antibacterial activity against propionibacterium acnes and staphylococcus epidermidis bacteria. This study provides new insights into the development of natural ingredient-based dermatological products that can be utilized in skin care.
Q: What is the purpose of this study?
A: The primary objective of this study is to develop a nanocrim formulation of ethanol extract from honey guava fruit and evaluate its antibacterial activity against propionibacterium acnes and staphylococcus epidermidis bacteria.
Q: What is the significance of this study?
A: This study provides new insights into the development of natural ingredient-based dermatological products that can be utilized in skin care. The results of this study demonstrate the potential of ethanol extract from honey guava fruit as a natural ingredient in the treatment of acne.
Q: What are the limitations of this study?
A: This study has some limitations. The study only evaluated the antibacterial activity of the nanocrim formulation against two types of bacteria, propionibacterium acnes and staphylococcus epidermidis. Further studies are needed to evaluate the antibacterial activity of the nanocrim formulation against other types of bacteria. Additionally, the study only evaluated the physical characteristics of the nanocrim formulation, and further studies are needed to evaluate the stability and shelf life of the nanocrim formulation.
Q: What are the potential applications of this study?
A: The results of this study demonstrate the potential of ethanol extract from honey guava fruit as a natural ingredient in the treatment of acne. This study provides new insights into the development of natural ingredient-based dermatological products that can be utilized in skin care.
Q: What are the future directions of this study?
A: Further studies are needed to evaluate the antibacterial activity of the nanocrim formulation against other types of bacteria and to evaluate the stability and shelf life of the nanocrim formulation. Additionally, further studies are needed to evaluate the efficacy of the nanocrim formulation in treating acne in humans.
Q: What are the benefits of using nanocrim-based honey guava fruit in the treatment of acne?
A: The nanocrim formulation of ethanol extract from honey guava fruit shows good physical characteristics and antibacterial activity against propionibacterium acnes and staphylococcus epidermidis bacteria. This demonstrates the great potential for using nanocrim-based honey guava fruit as an alternative in the treatment of acne that is more effective.
Q: What are the potential risks associated with using nanocrim-based honey guava fruit in the treatment of acne?
A: There are no reported risks associated with using nanocrim-based honey guava fruit in the treatment of acne. However, further studies are needed to evaluate the safety and efficacy of the nanocrim formulation in humans.
Q: How can I obtain the nanocrim formulation of ethanol extract from honey guava fruit?
A: The nanocrim formulation of ethanol extract from honey guava fruit is not commercially available. However, the results of this study provide new insights into the development of natural ingredient-based dermatological products that can be utilized in skin care.
Q: Can I use the nanocrim formulation of ethanol extract from honey guava fruit as a treatment for other skin conditions?
A: The results of this study demonstrate the potential of ethanol extract from honey guava fruit as a natural ingredient in the treatment of acne. However, further studies are needed to evaluate the efficacy of the nanocrim formulation in treating other skin conditions.
Q: How can I contact the researchers involved in this study?
A: The researchers involved in this study can be contacted through the university or research institution where the study was conducted.