Differences In The Effect Of Gotu Kola Leaf Methanol Extract (L.) Urban) On The Incidence Of Dysplasia Induced By 100 Μg And 200 Μg Diethylbenz (A) Anthracene (DMBA)

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Differences in the Effect of Gotu Kola Leaf Methanol Extract (Centella Asiatica (L.) Urban) on DMBA Induced Dysplasia

Oral cavity cancer is a type of head and neck cancer that has the highest death rate compared to all other types of malignancy. Epithelial dysplasia or epithelial dysplasia is a pre-cancer lesion that can be reversed into cancer when compared to lesions that do not experience dysplasia. Therefore, the level of epithelial dysplasia of the oral cavity can be an excellent predictor for assessing the risk of oral cancer. In an effort to prevent cancer, the use of natural or synthetic products to stop or reverse the process of malignant transformation is referred to as cancer chemoprevention.

One of the natural ingredients that is recommended as a chemopreventive agent is the Gepagan (Centella Asiatica (L.) Urban), which is known to have a low therapeutic effect on toxicity. This study aims to examine the difference in the effect of gotu kola leaf methanol extract on the event of dysplasia induced by Diethylbenz (A) Anthracene (DMBA) in mice. This research is a type of experimental laboratory research with a research sample consisting of 28 rats of wistar strains (Rattus norvegicus) which are divided into four groups.

Each group receives different treatment: the first group gets a mechanical leaf methanol extract and 100 µg DMBA 0.5%, the second group is given 100 µg DMBA 0.5% without extracts, the third group gets methanol extract and 200 µg DMBA 0.5%, and the fourth group was given 200 µg DMBA 0.5% without extract. The experiment was carried out for 61 days, where the mouse mucosa tissue was taken to be examined histopathologically to see the incidence of dysplasia. Dysplasia level is measured using WHO 2017 scores.

The Importance of Cancer Chemoprevention

Cancer chemoprevention is a crucial approach in preventing cancer. It involves the use of natural or synthetic products to stop or reverse the process of malignant transformation. This approach has gained significant attention in recent years due to its potential in reducing the risk of cancer. Gotu kola (Centella Asiatica (L.) Urban) is one of the natural ingredients that has been recommended as a chemopreventive agent. It is known to have a low therapeutic effect on toxicity, making it a safe option for cancer prevention.

The Role of Gotu Kola in Cancer Prevention

Gotu kola contains compounds such as Asiaticoside, Madecassoside, and Flavonoids which have anti-inflammatory and antioxidant properties. These compounds can help repair tissue and prevent cell damage that leads to dysplasia. Given that dysplasia is a condition that can be restored, interventions with gotu kola extract provide hope in the prevention of oral cancer.

The Effect of Gotu Kola Leaf Methanol Extract on DMBA Induced Dysplasia

The results of this study indicate that gotu kola leaf methanol extract can act as an effective chemopreventive agent in reducing the events of dysplasia induced by DMBA. Decreased levels of dysplasia in groups that get the extract indicate a protection mechanism that may be associated with bioactive components in gotu kola. The group given by the extract shows a lower level of dysplasia compared to groups that are not given extracts.

Analysis of the Results

The results of this study provide new insights in the cancer prevention approach. With the increasing number of evidence regarding the benefits of natural ingredients, further research needs to be done to determine the right dose and specific work mechanisms of gotu kola in preventing the development of cancer. The use of gotu kola leaves as an additional therapy choice in the prevention of oral cavity cancer is worth considering, given its potential ability to reduce the level of dysplasia and the possibility of transformation into cancer.

Conclusion

In conclusion, this study provides evidence that gotu kola leaf methanol extract can act as an effective chemopreventive agent in reducing the events of dysplasia induced by DMBA. The results of this study indicate that gotu kola extract may be a useful tool in preventing oral cancer. Further research is needed to determine the right dose and specific work mechanisms of gotu kola in preventing the development of cancer.

Recommendations

Based on the results of this study, the following recommendations are made:

  • Further research is needed to determine the right dose and specific work mechanisms of gotu kola in preventing the development of cancer.
  • Gotu kola leaves may be a useful tool in preventing oral cancer.
  • The use of gotu kola leaves as an additional therapy choice in the prevention of oral cavity cancer is worth considering.

Limitations of the Study

This study has several limitations. The sample size was small, and the study was conducted in a laboratory setting. Further research is needed to confirm the results of this study and to determine the effectiveness of gotu kola extract in preventing oral cancer in humans.

Future Directions

Future research should focus on determining the right dose and specific work mechanisms of gotu kola in preventing the development of cancer. Additionally, further research is needed to confirm the results of this study and to determine the effectiveness of gotu kola extract in preventing oral cancer in humans.
Q&A: Gotu Kola Leaf Methanol Extract and DMBA Induced Dysplasia

Q: What is Gotu Kola Leaf Methanol Extract?

A: Gotu kola leaf methanol extract is a natural ingredient that is derived from the leaves of the Centella Asiatica (L.) Urban plant. It has been traditionally used in medicine for its anti-inflammatory and antioxidant properties.

Q: What is DMBA Induced Dysplasia?

A: DMBA induced dysplasia is a condition that is caused by the exposure to Diethylbenz (A) Anthracene (DMBA), a chemical that is known to cause cancer. Dysplasia is a pre-cancerous condition that can be reversed into cancer if left untreated.

Q: What is the purpose of this study?

A: The purpose of this study is to examine the effect of gotu kola leaf methanol extract on DMBA induced dysplasia in mice. The study aims to determine whether gotu kola extract can act as an effective chemopreventive agent in reducing the events of dysplasia induced by DMBA.

Q: What were the results of the study?

A: The results of the study indicate that gotu kola leaf methanol extract can act as an effective chemopreventive agent in reducing the events of dysplasia induced by DMBA. The group given by the extract shows a lower level of dysplasia compared to groups that are not given extracts.

Q: What are the potential benefits of using gotu kola extract in cancer prevention?

A: The potential benefits of using gotu kola extract in cancer prevention include its ability to reduce the level of dysplasia and the possibility of transformation into cancer. Gotu kola extract may also have anti-inflammatory and antioxidant properties that can help repair tissue and prevent cell damage.

Q: What are the limitations of the study?

A: The limitations of the study include the small sample size and the fact that the study was conducted in a laboratory setting. Further research is needed to confirm the results of this study and to determine the effectiveness of gotu kola extract in preventing oral cancer in humans.

Q: What are the future directions for research?

A: Future research should focus on determining the right dose and specific work mechanisms of gotu kola in preventing the development of cancer. Additionally, further research is needed to confirm the results of this study and to determine the effectiveness of gotu kola extract in preventing oral cancer in humans.

Q: Can gotu kola extract be used as a treatment for oral cancer?

A: While gotu kola extract may have potential benefits in cancer prevention, it is not a treatment for oral cancer. Further research is needed to determine its effectiveness as a treatment for oral cancer.

Q: Are there any side effects associated with the use of gotu kola extract?

A: There are no reported side effects associated with the use of gotu kola extract. However, further research is needed to determine its safety and efficacy in humans.

Q: How can I obtain gotu kola extract?

A: Gotu kola extract can be obtained from health food stores or online retailers. However, it is essential to consult with a healthcare professional before using any new supplement.

Q: Can I use gotu kola extract in combination with other cancer treatments?

A: It is essential to consult with a healthcare professional before using gotu kola extract in combination with other cancer treatments. While gotu kola extract may have potential benefits in cancer prevention, it is not a substitute for conventional cancer treatments.