Bioactivity Analysis Of Irrigation Materials Lerak Fruit Extract (Sapindus Rarak DC) To Pathogenesis Of Fusobacterium Nucleatum (Anti-Adhesion Test Study, Biomass Test, And Test Porosity Of Tooth Root Channels) (In Vitro Research)
Bioactivity Analysis of Lerak Fruit Extract (Sapindus Rarak DC) to the Pathogenesis of Fusobacterium Nucleatum: In Vitro Test
Introduction
Endodontic infections are a common problem in dentistry, often caused by various types of bacteria, including fusobacterium nucleatum. These bacteria are involved in the formation of biofilm, which can worsen the condition of infection in the root canal. In the practice of dentistry, this infection is usually overcome through a procedure of chemical and mechanical cleaning and shaping. However, the use of sodium hypochlorite (NaOCl) has a significant weakness, namely cytotoxicity when this solution is extracted to the periradicular tissue, as well as the potential to cause erosion in the dentin.
The Need for Alternative Irrigation Materials
Anticipating the weaknesses of this irrigation material, research has found that the use of natural ingredients can be a more biocompatible alternative. One of the natural ingredients that gets attention is the lerak fruit extract (Sapindus Rarak DC). This study aims to evaluate the bioactivity of lerak fruit extract against fusobacterium nucleatum with a focus on the anti-adhesion test, biomass, and porosity of the root canal walls.
Research Methodology
In this study, the bacterial colony fusobacterium nucleatum was bred using Crystal Violet Erythromycin (CVE) media. Lerak fruit extract is used in a concentration variation of 6.25%, 12.5%, and 25%, compared to 2.5% NaOCl solution and 17% EDTA. This study involved observations for 24, 48, and 72 hours to measure its ability to prevent adhesion, inhibit biomass growth, and evaluate the porosity of the walls of the tooth root canal.
Results and Analysis
Statistical analysis conducted using the Kruskal-Wallis and One Way Anova test, as well as further tests such as LSD and Mann-Whitney, showed interesting results. Lerak fruit extract with various concentrations (6.25%, 12.5%, and 25%) proved effective in preventing adhesion and inhibiting the development of bacteria fusobacterium nucleatum, equivalent to 2.5% NaOCl solution and 17% EDTA which became Gold Standard in root canal irrigation.
From the results of the study, it is known that although the NaOCL solution 2.5% and EDTA 17% show a higher level of porosity, these results must also be considered in the context of long-term use. In other words, although high porosity can increase cleaning, the risk of damage to dentin also needs to be aware of.
This lerak fruit extract, at the level of concentration being tested, does not show a significant increase in porosity, making it a safe and efficient material for use in irrigation procedures. This implies that lerak fruit extract can be a better choice, given its safety to dental tissue while maintaining effectiveness as an antimicrobial agent.
Conclusion
This study shows that lerak fruit extract (Sapindus Rarak DC) has the potential as an effective natural irrigation ingredient in dealing with endodontic infections caused by fusobacterium nucleatum. With the ability to prevent adhesion and inhibit the development of bacteria, and does not significantly increase porosity, lerak extract offers alternative solutions that meet the aspects of biocompatibility that are needed in dental practice. Thus, the use of natural ingredients such as lerak fruit extract is increasingly relevant in efforts to develop a safer and effective treatment method for dental and oral health.
Implications of the Study
The results of this study have significant implications for the development of new irrigation materials for endodontic infections. The use of natural ingredients such as lerak fruit extract can provide a safer and more effective alternative to traditional irrigation materials. This study highlights the potential of lerak fruit extract as a biocompatible and effective irrigation material, and suggests that further research is needed to explore its potential in clinical settings.
Future Directions
Future studies should aim to investigate the long-term effects of lerak fruit extract on dental tissue and its potential to cause damage to dentin. Additionally, further research is needed to explore the potential of lerak fruit extract as a treatment for other types of bacterial infections. The use of natural ingredients such as lerak fruit extract has the potential to revolutionize the treatment of endodontic infections and provide a safer and more effective alternative to traditional irrigation materials.
Limitations of the Study
This study has several limitations that should be considered when interpreting the results. The study was conducted in vitro, and further research is needed to explore the potential of lerak fruit extract in clinical settings. Additionally, the study only investigated the effects of lerak fruit extract on fusobacterium nucleatum, and further research is needed to explore its potential against other types of bacteria.
Conclusion
In conclusion, this study shows that lerak fruit extract (Sapindus Rarak DC) has the potential as an effective natural irrigation ingredient in dealing with endodontic infections caused by fusobacterium nucleatum. With the ability to prevent adhesion and inhibit the development of bacteria, and does not significantly increase porosity, lerak extract offers alternative solutions that meet the aspects of biocompatibility that are needed in dental practice. Thus, the use of natural ingredients such as lerak fruit extract is increasingly relevant in efforts to develop a safer and effective treatment method for dental and oral health.
Q&A: Bioactivity Analysis of Lerak Fruit Extract (Sapindus Rarak DC) to the Pathogenesis of Fusobacterium Nucleatum
Frequently Asked Questions
Q: What is the purpose of this study?
A: The purpose of this study is to evaluate the bioactivity of lerak fruit extract against fusobacterium nucleatum with a focus on the anti-adhesion test, biomass, and porosity of the root canal walls.
Q: What is fusobacterium nucleatum?
A: Fusobacterium nucleatum is a type of bacteria that is commonly found in the oral cavity and is involved in the formation of biofilm, which can worsen the condition of infection in the root canal.
Q: What are the limitations of using NaOCl as an irrigation material?
A: The use of NaOCl has a significant weakness, namely cytotoxicity when this solution is extracted to the periradicular tissue, as well as the potential to cause erosion in the dentin.
Q: What are the benefits of using lerak fruit extract as an irrigation material?
A: Lerak fruit extract has been shown to be effective in preventing adhesion and inhibiting the development of bacteria fusobacterium nucleatum, equivalent to 2.5% NaOCl solution and 17% EDTA. Additionally, it does not show a significant increase in porosity, making it a safe and efficient material for use in irrigation procedures.
Q: What are the implications of this study for the development of new irrigation materials?
A: The results of this study have significant implications for the development of new irrigation materials for endodontic infections. The use of natural ingredients such as lerak fruit extract can provide a safer and more effective alternative to traditional irrigation materials.
Q: What are the potential applications of lerak fruit extract in dentistry?
A: Lerak fruit extract has the potential to be used as a treatment for endodontic infections caused by fusobacterium nucleatum. It may also be used as a treatment for other types of bacterial infections.
Q: What are the potential risks associated with the use of lerak fruit extract?
A: The potential risks associated with the use of lerak fruit extract are not well understood and further research is needed to explore its potential long-term effects on dental tissue.
Q: What are the next steps in the development of lerak fruit extract as an irrigation material?
A: Further research is needed to explore the potential of lerak fruit extract in clinical settings and to investigate its long-term effects on dental tissue.
Q: How can lerak fruit extract be used in dentistry?
A: Lerak fruit extract can be used as an irrigation material in endodontic procedures to prevent adhesion and inhibit the development of bacteria fusobacterium nucleatum.
Q: What are the benefits of using natural ingredients in dentistry?
A: The use of natural ingredients such as lerak fruit extract can provide a safer and more effective alternative to traditional irrigation materials.
Q: What are the potential benefits of using lerak fruit extract in combination with other natural ingredients?
A: The potential benefits of using lerak fruit extract in combination with other natural ingredients are not well understood and further research is needed to explore its potential.
Q: What are the potential applications of lerak fruit extract in other fields?
A: The potential applications of lerak fruit extract in other fields are not well understood and further research is needed to explore its potential.
Conclusion
In conclusion, this study shows that lerak fruit extract (Sapindus Rarak DC) has the potential as an effective natural irrigation ingredient in dealing with endodontic infections caused by fusobacterium nucleatum. With the ability to prevent adhesion and inhibit the development of bacteria, and does not significantly increase porosity, lerak extract offers alternative solutions that meet the aspects of biocompatibility that are needed in dental practice.