Application And Validation Of Ultra Violet Spectrophotometry Method Spectrophotometry Derivatives And Mean Centering Of Ratio Spectra To Ternary Mixture Containing Paracetamol, Propifenazone And Cofeine

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Application and Validation of Ultra Violet Spectrophotometry Method to Determine Paracetamol, Propifenazone, and Cofein Paracetamol Ternary Mixtures Levels

In the pharmaceutical industry, the development of new drugs and formulations is a continuous process. Many drug preparations on the market contain more than one active substance, such as analgesic drugs that combine paracetamol, propifenazone, and cofeine in the form of a mixture of Ternary. This study aims to develop and validate spectrophotometric methods, including zero-crossing derivative techniques, zero-crossing ratios, and mean centering of ratio spectra, to determine the levels of the three substances in tablet preparations without the need to separate.

Research Methodology

This study begins with optimizing the type of solvent used, namely phosphate buffer with a pH of 7.2, HCl 0.1 N, and a mixture of phosphate pH 7.2 with methanol in various comparisons (90:10, 70:30, 50:50 , 30:70, and 10:90). The spectrophotometry method with the zero-crossing technique, the zero-crossing ratio, and the mean centering of the spectra ratio then tested its validity based on several parameters, such as linearity, accuracy, precision, limit of detection (LOD), and the limit of quantification (LOQ).

The results showed that the combination of phosphate pH 7.2 and methanol with a ratio of 70:30 was the most effective solvent for levels of levels. This finding is crucial in the development of spectrophotometric methods, as the choice of solvent can significantly affect the accuracy and precision of the results.

Results and Discussion

In the application of zero-crossing techniques, paracetamol and cofeine levels are determined through the first derivative at a wavelength of 239.4 nm and 245.6 nm, while propifenazone is measured at the third derivative at a wavelength of 249.6 nm. The analysis showed that the level of each substance was 100.91% for paracetamol, 104.75% for propifenazone, and 103.33% for cofeine. These results indicate that the zero-crossing technique is a reliable method for determining the levels of these three substances.

With the zero-crossing ratio technique, the levels of these three active substances can also be determined in the first derivative. Measurements were made at wavelengths 274.0 Nm for paracetamol, 315.2 Nm for propifenazone, and 269.2 Nm for cofeine, with successive levels of 100.11%, 102.38%, and 100.59%. The results obtained from this technique are comparable to those obtained from the zero-crossing technique, indicating that the zero-crossing ratio technique is also a reliable method for determining the levels of these substances.

Meanwhile, in the Mean Centering of Ratio Spectra method, Paracetamol, Propifenazone, and Cofein levels are determined at a wavelength of 244.0 nm, 272.0 nm, and 273.0 nm, producing levels of 105.49%, 99, 76%, and 99.64%. The results obtained from this technique are slightly different from those obtained from the zero-crossing and zero-crossing ratio techniques, indicating that the Mean Centering of Ratio Spectra method may be more sensitive to the presence of other substances in the mixture.

Conclusion

Based on the results of this study, it can be concluded that the three spectrophotometric methods tested-zero-crossing derivatives, zero-crossing ratios, and mean centering of ratio spectra-meet the validation requirements of the set method. With optimal use of solvents and appropriate analysis methods, paracetamol, propifenazone, and cofeine levels in tablet preparations can be determined accurately and efficiently. This research not only contributes to the development of analytical methods, but also opens the way for further research regarding the determination of the level of active substances in other complex preparations.

Implications of the Study

The findings of this study have significant implications for the pharmaceutical industry. The development of spectrophotometric methods that can accurately determine the levels of multiple active substances in a single mixture is a major breakthrough. This method can be used to determine the levels of paracetamol, propifenazone, and cofeine in tablet preparations, which can help to ensure the quality and efficacy of these products.

Future Directions

This study opens the way for further research regarding the determination of the level of active substances in other complex preparations. The development of spectrophotometric methods that can accurately determine the levels of multiple active substances in a single mixture is a major breakthrough, and this research can be used as a starting point for further studies.

Limitations of the Study

This study has several limitations. The use of a single solvent system may not be representative of all possible solvent systems, and the results obtained from this study may not be generalizable to other solvent systems. Additionally, the study only tested the three spectrophotometric methods on a single mixture of paracetamol, propifenazone, and cofeine, and the results may not be applicable to other mixtures.

Recommendations for Future Research

Based on the findings of this study, several recommendations can be made for future research. Firstly, the study should be replicated using different solvent systems to determine the effect of solvent on the accuracy and precision of the results. Secondly, the study should be extended to include other mixtures of active substances to determine the applicability of the spectrophotometric methods to different types of mixtures. Finally, the study should be used as a starting point for further research regarding the development of spectrophotometric methods for determining the levels of active substances in complex preparations.

Conclusion

In conclusion, this study has demonstrated the effectiveness of three spectrophotometric methods-zero-crossing derivatives, zero-crossing ratios, and mean centering of ratio spectra-in determining the levels of paracetamol, propifenazone, and cofeine in tablet preparations. The results obtained from this study have significant implications for the pharmaceutical industry, and this research can be used as a starting point for further studies.
Frequently Asked Questions (FAQs) about Application and Validation of Ultra Violet Spectrophotometry Method to Determine Paracetamol, Propifenazone, and Cofein Paracetamol Ternary Mixtures Levels

Q: What is the purpose of this study?

A: The purpose of this study is to develop and validate spectrophotometric methods, including zero-crossing derivative techniques, zero-crossing ratios, and mean centering of ratio spectra, to determine the levels of paracetamol, propifenazone, and cofeine in tablet preparations without the need to separate.

Q: What are the benefits of using spectrophotometric methods to determine the levels of active substances in tablet preparations?

A: The benefits of using spectrophotometric methods to determine the levels of active substances in tablet preparations include accuracy, precision, and efficiency. These methods can also help to ensure the quality and efficacy of the products.

Q: What are the limitations of this study?

A: The limitations of this study include the use of a single solvent system, which may not be representative of all possible solvent systems, and the results obtained from this study may not be generalizable to other solvent systems. Additionally, the study only tested the three spectrophotometric methods on a single mixture of paracetamol, propifenazone, and cofeine, and the results may not be applicable to other mixtures.

Q: What are the implications of this study for the pharmaceutical industry?

A: The findings of this study have significant implications for the pharmaceutical industry. The development of spectrophotometric methods that can accurately determine the levels of multiple active substances in a single mixture is a major breakthrough. This method can be used to determine the levels of paracetamol, propifenazone, and cofeine in tablet preparations, which can help to ensure the quality and efficacy of these products.

Q: What are the future directions for this research?

A: The future directions for this research include replicating the study using different solvent systems to determine the effect of solvent on the accuracy and precision of the results, extending the study to include other mixtures of active substances to determine the applicability of the spectrophotometric methods to different types of mixtures, and using the study as a starting point for further research regarding the development of spectrophotometric methods for determining the levels of active substances in complex preparations.

Q: What are the recommendations for future research?

A: The recommendations for future research include:

  • Replicating the study using different solvent systems to determine the effect of solvent on the accuracy and precision of the results.
  • Extending the study to include other mixtures of active substances to determine the applicability of the spectrophotometric methods to different types of mixtures.
  • Using the study as a starting point for further research regarding the development of spectrophotometric methods for determining the levels of active substances in complex preparations.

Q: What are the potential applications of this research?

A: The potential applications of this research include:

  • Determining the levels of paracetamol, propifenazone, and cofeine in tablet preparations.
  • Developing spectrophotometric methods for determining the levels of active substances in complex preparations.
  • Improving the quality and efficacy of pharmaceutical products.

Q: What are the potential benefits of this research?

A: The potential benefits of this research include:

  • Improving the accuracy and precision of spectrophotometric methods for determining the levels of active substances in complex preparations.
  • Enhancing the quality and efficacy of pharmaceutical products.
  • Reducing the time and cost associated with determining the levels of active substances in complex preparations.

Q: What are the potential limitations of this research?

A: The potential limitations of this research include:

  • The use of a single solvent system, which may not be representative of all possible solvent systems.
  • The results obtained from this study may not be generalizable to other solvent systems.
  • The study only tested the three spectrophotometric methods on a single mixture of paracetamol, propifenazone, and cofeine, and the results may not be applicable to other mixtures.