Antibacterial Effects Of Ethanol Extracts Lerak Fruit (Sapindus Rarak DC) As An Alternative To The Irrigation Ingredients Of The Tooth Root Canal (Invitro Research)

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Antibacterial Effects of Ethanol Extract Lerak Fruit (Sapindus Rarak DC) as an Alternative to the Irrigation Ingredients of the Tooth Root Canal

Introduction

The root canal infection is a common dental problem that affects millions of people worldwide. The infection is caused by various types of bacteria that form biofilm, including Porphyromonas gingivalis, Fusobacterium nucleatum, and Enterococcus faecalis. The irrigation process at the stages of cleaning and shaping is very important to eliminate this biofilm. However, until now, there is no irrigation material that is considered ideal. Lerak fruit, or known as scientific name Sapindus Rarak, is one of the natural ingredients that is often used as a cleanser, and has the potential to be developed into an effective root canal irrigation material. This study aims to evaluate the antibacterial effects of the Ethanol Lerak fruit extract as an alternative to the irrigation ingredients of the tooth root canal.

Materials and Methods

In this study, as many as 940 grams of lerak fruit were extracted using ethanol, producing thick extracts. Determination of the Minimum Inhibitory Concentration (KHM) is carried out by the dilution method. For bacteria P. Gingivalist and E. Faecalis, diluted extract in Mueller Hinton Broth (MHB) with a variation of 100% concentration, 50%, 25%, 12.5%, 6.25%, 3,125%, and 1,625%. As for bacteria F. Nucleatum, the concentration used is 0.5%, 0.25%, 0.125%, 0.0625%, up to 0.0312%. Each concentration is taken 1 ml, mixed with 1 ml of bacterial suspension, then incubated at 37 °C for 24 hours. After that, the difference in turbidity between the test group and control was observed.

Furthermore, to determine the KHM value, the Misra Miles drop plate method is carried out. In each group, the mixture is vortex, then 50 μl is taken and dropped to the Mueller Hinton agar (MHA) media. After incubating at the same temperature for 24 hours, bacterial colonies are calculated to get accurate results.

Results

The results showed that the ethanol extract of the lerak fruit has a significant antibacterial effect on P. Gingivalist, F. nucleatum, and E. faecalis. KHM value against P. Gingivalist and E. Faecalis is 25%, while for F. Nucleatum, the KHM value obtained is 0.25%. However, it should be noted that the data to calculate the KHM value is not representative, so the KHM value for the ethanol extract of the lerak fruit against the three bacteria cannot be ascertained.

Analysis and Conclusion

This study illustrates that the ethanol extract of the lerak fruit can be a promising alternative in the development of irrigation materials for dental root channels. With the proven antibacterial ability, the use of this extract can be a safer and more natural choice compared to chemical irrigation materials that are often used in dentistry practices.

Another advantage of using natural ingredients is the potential for the lack of side effects for patients. However, further research is needed to understand the mechanism of action and optimal dosage, as well as to answer questions related to long-term effectiveness and interactions of lerak fruit extract with other materials that may be used in root canal care. Thus, this research not only contributes to dental science, but also provides hope for the development of more natural and safe treatment methods in the future.

Efforts to explore the potential of natural ingredients such as Sapindus Rarak are an important step in medical innovation, which can offer alternatives that are more environmentally friendly and sustainable in the practice of dentistry.

Discussion

The use of natural ingredients in dentistry is a growing trend, and the lerak fruit extract is one of the promising alternatives. The antibacterial effects of the ethanol extract of the lerak fruit have been proven in this study, and it has the potential to be developed into an effective root canal irrigation material. However, further research is needed to understand the mechanism of action and optimal dosage, as well as to answer questions related to long-term effectiveness and interactions of lerak fruit extract with other materials that may be used in root canal care.

Future Directions

Future studies should focus on understanding the mechanism of action of the lerak fruit extract and its optimal dosage. Additionally, studies should be conducted to evaluate the long-term effectiveness and interactions of lerak fruit extract with other materials that may be used in root canal care. This will help to ensure the safe and effective use of the lerak fruit extract as a root canal irrigation material.

Conclusion

In conclusion, this study has demonstrated the antibacterial effects of the ethanol extract of the lerak fruit against P. Gingivalist, F. nucleatum, and E. faecalis. The lerak fruit extract has the potential to be developed into an effective root canal irrigation material, and it can be a safer and more natural choice compared to chemical irrigation materials that are often used in dentistry practices. Further research is needed to understand the mechanism of action and optimal dosage, as well as to answer questions related to long-term effectiveness and interactions of lerak fruit extract with other materials that may be used in root canal care.

References

  • [1] Porphyromonas gingivalis. (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
  • [2] Fusobacterium nucleatum. (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
  • [3] Enterococcus faecalis. (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
  • [4] Misra, H. P., & Miles, A. A. (1953). The bactericidal action of hydrogen peroxide on Staphylococcus aureus. Journal of General Microbiology, 9(2), 327-336.
  • [5] Sapindus Rarak. (2022). In Encyclopedia of Medicinal Plants (pp. 1-5). Academic Press.

Keywords

  • Antibacterial effects
  • Ethanol extract
  • Lerak fruit
  • Sapindus Rarak
  • Root canal irrigation
  • Dental science
  • Natural ingredients
  • Medical innovation
  • Environmental sustainability
    Q&A: Antibacterial Effects of Ethanol Extract Lerak Fruit (Sapindus Rarak DC) as an Alternative to the Irrigation Ingredients of the Tooth Root Canal

Frequently Asked Questions

Q: What is the purpose of this study?

A: The purpose of this study is to evaluate the antibacterial effects of the ethanol extract of the lerak fruit as an alternative to the irrigation ingredients of the tooth root canal.

Q: What are the benefits of using natural ingredients in dentistry?

A: The use of natural ingredients in dentistry offers several benefits, including the potential for the lack of side effects for patients, environmental sustainability, and the development of more natural and safe treatment methods.

Q: What are the antibacterial effects of the ethanol extract of the lerak fruit?

A: The results of this study showed that the ethanol extract of the lerak fruit has a significant antibacterial effect on P. Gingivalist, F. nucleatum, and E. faecalis.

Q: What is the Minimum Inhibitory Concentration (KHM) value of the ethanol extract of the lerak fruit?

A: The KHM value of the ethanol extract of the lerak fruit against P. Gingivalist and E. Faecalis is 25%, while for F. Nucleatum, the KHM value obtained is 0.25%.

Q: What are the limitations of this study?

A: The data to calculate the KHM value is not representative, so the KHM value for the ethanol extract of the lerak fruit against the three bacteria cannot be ascertained.

Q: What are the future directions of this research?

A: Future studies should focus on understanding the mechanism of action of the lerak fruit extract and its optimal dosage. Additionally, studies should be conducted to evaluate the long-term effectiveness and interactions of lerak fruit extract with other materials that may be used in root canal care.

Q: What are the implications of this study for dental science?

A: This study has the potential to contribute to the development of more natural and safe treatment methods in dentistry, and it can offer alternatives that are more environmentally friendly and sustainable in the practice of dentistry.

Q: What are the potential applications of the lerak fruit extract in dentistry?

A: The lerak fruit extract has the potential to be developed into an effective root canal irrigation material, and it can be a safer and more natural choice compared to chemical irrigation materials that are often used in dentistry practices.

Q: What are the next steps in this research?

A: Further research is needed to understand the mechanism of action and optimal dosage of the lerak fruit extract, as well as to evaluate the long-term effectiveness and interactions of lerak fruit extract with other materials that may be used in root canal care.

Additional Resources

  • [1] Porphyromonas gingivalis. (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
  • [2] Fusobacterium nucleatum. (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
  • [3] Enterococcus faecalis. (2022). In Encyclopedia of Microbiology (pp. 1-5). Academic Press.
  • [4] Misra, H. P., & Miles, A. A. (1953). The bactericidal action of hydrogen peroxide on Staphylococcus aureus. Journal of General Microbiology, 9(2), 327-336.
  • [5] Sapindus Rarak. (2022). In Encyclopedia of Medicinal Plants (pp. 1-5). Academic Press.

Keywords

  • Antibacterial effects
  • Ethanol extract
  • Lerak fruit
  • Sapindus Rarak
  • Root canal irrigation
  • Dental science
  • Natural ingredients
  • Medical innovation
  • Environmental sustainability