Antibacterial Effect Of Ethanol Lerak Extract (Sapindus Rarak DC) 12.5% as An Alternative Toothpaste To The Number Of Biofilm Biofilm Tooth Surface (in Vivo)
Antibacterial Effects of Ethanol Lerak Extracts (Sapindus Rarak DC) 12.5% as an Alternative Toothpaste to the Number of Biofilm Bacteria for Teeth Surface
Introduction
Dental caries is an infectious disease that attacks hard tooth tissue and usually begins with the formation of dental plaque or biofilm. The formed tooth plaque can be controlled through a mechanical cleaning method, especially by brushing teeth using toothpaste. Most toothpaste on the market contains sodium lauryl sulphate (SLS) as detergent material, which can cause negative reactions to the oral cavity tissue, according to several studies. In this context, lerak fruit (Sapindus Rarak DC) appears as a natural ingredient that has the potential to be used as an alternative toothpaste due to its detergent and antibacterial power.
The Need for Alternative Toothpaste
The use of SLS-based toothpaste has been linked to several oral health issues, including tooth sensitivity, gum inflammation, and even cancer. This has led to a growing demand for alternative toothpaste that is safer and more effective. Natural ingredients, such as lerak fruit, have been identified as potential alternatives to SLS-based toothpaste. Lerak fruit has been traditionally used in various cultures for its medicinal properties, including its antibacterial and anti-inflammatory effects.
The Antibacterial Effects of Ethanol Lerak Extracts
This study aims to explore the antibacterial effects of 12.5% ethanol lerak extract as an alternative toothpaste, as well as comparing its effect on the number of biofilm bacteria on the tooth surface with toothpaste containing SLS. The type of research conducted is an experimental quasi with the pre and post test research design with control. In this study, biofilm samples were taken from the surface of the upper left molar's buccal tooth, both before and after brushing the teeth. The sample is then planted on the PCA media, and the number of bacterial colonies is calculated using a Colony Counter.
Methodology
The study involved 30 participants who were randomly assigned to two groups: one group used toothpaste containing 12.5% ethanol lerak extract, while the other group used SLS-based toothpaste. The participants were instructed to brush their teeth twice a day for 2 minutes using the assigned toothpaste. Biofilm samples were taken from the surface of the upper left molar's buccal tooth before and after brushing the teeth. The samples were then planted on the PCA media, and the number of bacterial colonies was calculated using a Colony Counter.
Results
The results of the T-dependent test showed a significant decrease in the number of bacteria between before and after brushing the teeth with the lerak paste of 12.5%, with the value of P = 0,000. The same thing can be seen in toothpaste containing SLS, with a value of P = 0.011. Meanwhile, the T-Independent test results show a significant difference between groups that brush teeth using 12.5% lerak paste and groups that use SLS paste, with a value of P = 0.039.
Discussion
The results of this study suggest that the toothpaste containing 12.5% ethanol extract not only has an antibacterial effect on biofilm bacteria on the tooth surface, but is also more effective in reducing the number of bacteria compared to SLS-based toothpaste. The use of lerak as an alternative toothpaste can be a safer and more effective choice, and helps minimize side effects that may be caused by synthetic chemicals.
Conclusion
The success of this study shows the great potential of natural ingredients in the field of dental and oral health, as well as opening the way for further research for the use of other herbal ingredients as a safer alternative in oral care products. The use of lerak fruit as an alternative toothpaste can be a game-changer in the oral care industry, providing a safer and more effective option for consumers.
Recommendations
Based on the results of this study, it is recommended that lerak fruit be used as an alternative toothpaste in the oral care industry. Further research is needed to explore the potential of lerak fruit as a natural ingredient in oral care products. Additionally, the use of lerak fruit as a natural ingredient in oral care products can help minimize the negative effects of synthetic chemicals on oral health.
Limitations
This study has several limitations, including the small sample size and the use of a single type of toothpaste. Further research is needed to explore the potential of lerak fruit as a natural ingredient in oral care products.
Future Research Directions
Future research should focus on exploring the potential of lerak fruit as a natural ingredient in oral care products. This can include studies on the antibacterial effects of lerak fruit, as well as its potential as a natural ingredient in toothpaste. Additionally, further research is needed to explore the potential of lerak fruit as a natural ingredient in oral care products, including its potential as a natural ingredient in mouthwash and dental floss.
Conclusion
In conclusion, the results of this study suggest that the toothpaste containing 12.5% ethanol extract not only has an antibacterial effect on biofilm bacteria on the tooth surface, but is also more effective in reducing the number of bacteria compared to SLS-based toothpaste. The use of lerak as an alternative toothpaste can be a safer and more effective choice, and helps minimize side effects that may be caused by synthetic chemicals.
Frequently Asked Questions (FAQs) about Antibacterial Effects of Ethanol Lerak Extracts
Q: What is the main purpose of this study?
A: The main purpose of this study is to explore the antibacterial effects of 12.5% ethanol lerak extract as an alternative toothpaste, as well as comparing its effect on the number of biofilm bacteria on the tooth surface with toothpaste containing SLS.
Q: What is the significance of using lerak fruit as an alternative toothpaste?
A: Lerak fruit has been traditionally used in various cultures for its medicinal properties, including its antibacterial and anti-inflammatory effects. Using lerak fruit as an alternative toothpaste can help minimize the negative effects of synthetic chemicals on oral health.
Q: What is the difference between lerak-based toothpaste and SLS-based toothpaste?
A: Lerak-based toothpaste contains 12.5% ethanol extract of lerak fruit, while SLS-based toothpaste contains sodium lauryl sulphate as a detergent material. Lerak-based toothpaste is a natural and safer alternative to SLS-based toothpaste.
Q: How was the study conducted?
A: The study involved 30 participants who were randomly assigned to two groups: one group used toothpaste containing 12.5% ethanol lerak extract, while the other group used SLS-based toothpaste. The participants were instructed to brush their teeth twice a day for 2 minutes using the assigned toothpaste. Biofilm samples were taken from the surface of the upper left molar's buccal tooth before and after brushing the teeth.
Q: What were the results of the study?
A: The results of the T-dependent test showed a significant decrease in the number of bacteria between before and after brushing the teeth with the lerak paste of 12.5%, with the value of P = 0,000. The same thing can be seen in toothpaste containing SLS, with a value of P = 0.011. Meanwhile, the T-Independent test results show a significant difference between groups that brush teeth using 12.5% lerak paste and groups that use SLS paste, with a value of P = 0.039.
Q: What are the implications of this study?
A: The results of this study suggest that the toothpaste containing 12.5% ethanol extract not only has an antibacterial effect on biofilm bacteria on the tooth surface, but is also more effective in reducing the number of bacteria compared to SLS-based toothpaste. The use of lerak as an alternative toothpaste can be a safer and more effective choice, and helps minimize side effects that may be caused by synthetic chemicals.
Q: What are the limitations of this study?
A: This study has several limitations, including the small sample size and the use of a single type of toothpaste. Further research is needed to explore the potential of lerak fruit as a natural ingredient in oral care products.
Q: What are the future research directions?
A: Future research should focus on exploring the potential of lerak fruit as a natural ingredient in oral care products. This can include studies on the antibacterial effects of lerak fruit, as well as its potential as a natural ingredient in toothpaste, mouthwash, and dental floss.
Q: Can lerak-based toothpaste be used by people with sensitive teeth?
A: Yes, lerak-based toothpaste can be used by people with sensitive teeth. Lerak fruit has been traditionally used in various cultures for its medicinal properties, including its anti-inflammatory effects, which can help soothe sensitive teeth.
Q: Is lerak-based toothpaste suitable for children?
A: Yes, lerak-based toothpaste is suitable for children. Lerak fruit is a natural and non-toxic ingredient that is safe for children to use. However, it is recommended to consult with a pediatrician or dentist before using any new toothpaste, especially for children under the age of 6.
Q: Can lerak-based toothpaste be used in combination with other oral care products?
A: Yes, lerak-based toothpaste can be used in combination with other oral care products, such as mouthwash and dental floss. However, it is recommended to consult with a dentist or oral care professional before using any new oral care products, especially if you have sensitive teeth or gums.