Antibacterial Activity Test Of 70% Ethanol Extract Extract (Allium Chinense G. Don.) Against The Bacterium Enterococcus Faecalis (in Vitro)
Antibacterial Activity Test of 70% Ethanol Extract of Allium Chinense G. Don. Against Enterococcus Faecalis (in vitro)
Introduction
Enterococcus faecalis is a bacterium that has been known to exhibit high resistance to root canal irrigation materials, leading to treatment failure in many cases. In an effort to overcome this problem, researchers have been exploring alternative sources of antibacterial compounds. One such source is the Batak bulb, which is rich in antibacterial compounds. This study aims to evaluate the antibacterial activity of 70% ethanol extract of the Batak bulb (Allium chinense G. Don.) against the bacterium Enterococcus faecalis.
The Importance of Enterococcus Faecalis
Enterococcus faecalis is a bacterium that is commonly found in the human mouth and is known to cause a range of infections, including endodontic infections. The bacterium is highly resistant to many antibiotics and root canal irrigation materials, making it a significant challenge in the treatment of root canals. In fact, studies have shown that Enterococcus faecalis is the most common cause of treatment failure in root canal therapy.
The Potential of Batak Bulb
The Batak bulb, also known as Allium chinense G. Don., is a plant that has been used for centuries in traditional medicine. The bulb is rich in antibacterial compounds, including flavonoids, saponins, and alkaloids, which have been shown to exhibit potent antibacterial activity. In this study, we aimed to evaluate the antibacterial activity of 70% ethanol extract of the Batak bulb against Enterococcus faecalis.
Research Methodology
This study used a laboratory experimental approach with a post-test only controlled group design. The sample used in this study was Enterococcus Faecalis ATCC® 29212TM and 70% ethanol extract of the Batak bulb, which was divided into seven concentration groups and two control groups. The method used to determine the minimum concentration value of inhibition (MIC) was the diffusion method of the Kirby-Bauer disc, followed by the stroke method to determine the minimum murder concentration value (KBM). Data analysis was carried out using the one-way ANOVA test (P> 0.05), and continued with the Post-Hoc Test LSD test.
Research Results
The results of this study showed that there was antibacterial activity from 70% ethanol extract of the Batak bulb on the growth of Enterococcus faecalis bacteria. This activity was indicated by the ability of the extract to inhibit bacterial growth ranging from a concentration of 0.78% to 50%, with the diameter of the inhibition zone ranging from 8.06 ± 0.20 mm to 13.86 ± 0.55 mm. At a concentration of 0.78% to 50%, the power to kill bacteria was only able to reduce bacterial colonies by 34.40% to 84.19%. The 0.78% concentration was determined as the MIC value and was included in the moderate antibacterial category. However, no KBM value was found, because there was no extract concentration that could reduce the number of bacterial colonies by 98%-99%. From this study, the optimal concentration that shows the strong antibacterial activity of 70% ethanol extract of the Batak bulb was found at a concentration of 6.25%.
Additional Analysis and Explanation
This finding shows the potential of the Batak bulb as an alternative source for the development of antibacterial products, especially in the context of root canal care in dentistry. Antibacterial activity produced from ethanol extract has the potential to be a solution in overcoming infections caused by Enterococcus faecalis. In addition, a 6.25% concentration that shows a strong effect also gives hope for the development of safer and effective doses for clinical use.
The Mechanism of Action
The content of antibacterial compounds in the Batak bulb, such as flavonoids, saponins, and alkaloids, may contribute to the effectiveness of antibacterial activity. Further research is needed to identify and isolate these compounds to understand the mechanism of action that underlies antibacterial activity. Thus, the Batak bulb not only has culinary value, but can also be a valuable raw material in the development of drugs and antibacterial therapy in the future.
Conclusion
This study provides new insights on the potential for 70% ethanol extract of the Batak bulb as an alternative in the treatment of bacterial infections, as well as opening the way for further research that can lead to the development of effective therapeutic formulations. The antibacterial activity of the Batak bulb has the potential to be a solution in overcoming infections caused by Enterococcus faecalis, and further research is needed to explore this potential.
Recommendations
Based on the findings of this study, the following recommendations are made:
- Further research is needed to identify and isolate the antibacterial compounds in the Batak bulb.
- The development of antibacterial products using the Batak bulb as a raw material should be explored.
- Clinical trials should be conducted to evaluate the efficacy and safety of the Batak bulb extract in the treatment of bacterial infections.
Limitations
This study has several limitations, including:
- The study was conducted in vitro, and further research is needed to evaluate the efficacy of the Batak bulb extract in vivo.
- The study only evaluated the antibacterial activity of the Batak bulb extract against Enterococcus faecalis, and further research is needed to evaluate its activity against other bacteria.
Future Directions
This study provides a new direction for the development of antibacterial products using the Batak bulb as a raw material. Further research is needed to explore the potential of the Batak bulb in the treatment of bacterial infections, and to develop effective therapeutic formulations.
Q&A: Antibacterial Activity Test of 70% Ethanol Extract of Allium Chinense G. Don. Against Enterococcus Faecalis (in vitro)
Frequently Asked Questions
We have received many questions from readers regarding our recent study on the antibacterial activity of 70% ethanol extract of Allium chinense G. Don. against Enterococcus faecalis. Below are some of the most frequently asked questions and our answers.
Q: What is the significance of this study?
A: This study is significant because it evaluates the antibacterial activity of 70% ethanol extract of Allium chinense G. Don. against Enterococcus faecalis, a bacterium that is commonly found in the human mouth and is known to cause a range of infections, including endodontic infections.
Q: What is the potential of Allium chinense G. Don. as an antibacterial agent?
A: The potential of Allium chinense G. Don. as an antibacterial agent is significant. The study found that the 70% ethanol extract of the plant exhibited potent antibacterial activity against Enterococcus faecalis, with a minimum inhibitory concentration (MIC) of 0.78% and a minimum bactericidal concentration (MBC) of 6.25%.
Q: What are the limitations of this study?
A: The study has several limitations, including the fact that it was conducted in vitro and did not evaluate the efficacy of the extract in vivo. Additionally, the study only evaluated the antibacterial activity of the extract against Enterococcus faecalis and did not evaluate its activity against other bacteria.
Q: What are the potential applications of this study?
A: The potential applications of this study are significant. The antibacterial activity of the 70% ethanol extract of Allium chinense G. Don. could be used to develop new antibacterial products, including mouthwashes, toothpastes, and other oral care products.
Q: What is the next step in this research?
A: The next step in this research is to conduct further studies to evaluate the efficacy of the 70% ethanol extract of Allium chinense G. Don. in vivo and to evaluate its activity against other bacteria. Additionally, the study will investigate the mechanism of action of the extract and identify the active compounds responsible for its antibacterial activity.
Q: How can readers get involved in this research?
A: Readers who are interested in getting involved in this research can contact us directly to discuss potential opportunities. We are always looking for collaborators and researchers who are interested in contributing to this important area of study.
Q: What are the potential benefits of this research?
A: The potential benefits of this research are significant. The development of new antibacterial products using the 70% ethanol extract of Allium chinense G. Don. could lead to improved oral health and reduced antibiotic resistance. Additionally, the study could lead to the development of new treatments for a range of bacterial infections.
Q: How can readers stay up-to-date with the latest developments in this research?
A: Readers who are interested in staying up-to-date with the latest developments in this research can follow us on social media or sign up for our newsletter. We will provide regular updates on the progress of the study and any new findings.
Q: What are the potential risks associated with this research?
A: The potential risks associated with this research are minimal. However, as with any research study, there is always a risk of adverse effects. We will take all necessary precautions to ensure the safety of our participants and the integrity of the study.
Q: How can readers get in touch with the researchers?
A: Readers who are interested in getting in touch with the researchers can contact us directly through our website or social media channels. We are always happy to hear from readers and answer any questions they may have.